{"id":2936,"date":"2026-02-24T19:04:24","date_gmt":"2026-02-24T11:04:24","guid":{"rendered":"http:\/\/www.majunpeng.com\/?p=2936"},"modified":"2026-02-24T19:59:22","modified_gmt":"2026-02-24T11:59:22","slug":"%e5%9f%ba%e4%ba%8etrinity%e8%bf%9b%e8%a1%8c%e6%97%a0%e5%8f%82%e8%bd%ac%e5%bd%95%e7%bb%84%e7%9a%84%e7%bb%84%e8%a3%85","status":"publish","type":"post","link":"http:\/\/www.majunpeng.com\/index.php\/2026\/02\/24\/%e5%9f%ba%e4%ba%8etrinity%e8%bf%9b%e8%a1%8c%e6%97%a0%e5%8f%82%e8%bd%ac%e5%bd%95%e7%bb%84%e7%9a%84%e7%bb%84%e8%a3%85\/","title":{"rendered":"\u57fa\u4e8eTrinity\u8fdb\u884c\u65e0\u53c2\u8f6c\u5f55\u7ec4\u7684\u7ec4\u88c5"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2936\" class=\"elementor elementor-2936\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f94773f e-flex e-con-boxed e-con e-parent\" data-id=\"f94773f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-323b6c4 elementor-widget elementor-widget-heading\" data-id=\"323b6c4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u5f15\u8a00<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bbef67e elementor-widget elementor-widget-text-editor\" data-id=\"bbef67e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"0\" data-end=\"195\">\u968f\u7740 RNA-seq \u6280\u672f\u7684\u666e\u53ca\uff0c\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u5df2\u6210\u4e3a\u89e3\u6790\u57fa\u56e0\u8868\u8fbe\u4e0e\u529f\u80fd\u57fa\u56e0\u7ec4\u6210\u5206\u7684\u6838\u5fc3\u624b\u6bb5\u3002\u5bf9\u4e8e\u7f3a\u4e4f\u9ad8\u8d28\u91cf\u53c2\u8003\u57fa\u56e0\u7ec4\u7684\u7269\u79cd\uff0c\u65e0\u53c2\u8003\u8f6c\u5f55\u7ec4\u7ec4\u88c5\uff08de novo transcriptome assembly\uff09\u901a\u8fc7\u5bf9 RNA-seq reads \u8fdb\u884c\u56fe\u7ed3\u6784\u91cd\u5efa\uff0c\u76f4\u63a5\u6062\u590d\u8f6c\u5f55\u672c\u5e8f\u5217\uff0c\u4ece\u800c\u83b7\u5f97\u5b8c\u6574\u7684\u8f6c\u5f55\u672c\u96c6\u5408\u3002\u8fd9\u4e00\u7b56\u7565\u5e7f\u6cdb\u5e94\u7528\u4e8e\u975e\u6a21\u5f0f\u7269\u79cd\u3001\u591a\u500d\u4f53\u7269\u79cd\u53ca\u57fa\u56e0\u7ec4\u8d44\u6e90\u532e\u4e4f\u7684\u7c7b\u7fa4\uff0c\u662f\u5f00\u5c55\u5206\u5b50\u7814\u7a76\u7684\u91cd\u8981\u57fa\u7840\u5728\u6bd4\u8f83\u57fa\u56e0\u7ec4\u5c42\u9762\uff0c\u8f6c\u5f55\u7ec4\u6570\u636e\u80fd\u591f\u63d0\u4f9b\u9ad8\u8d28\u91cf\u7684\u7f16\u7801\u5e8f\u5217\uff0c\u7528\u4e8e\u540c\u6e90\u57fa\u56e0\u9274\u5b9a\u3001\u57fa\u56e0\u5bb6\u65cf\u6269\u5f20\u4e0e\u6536\u7f29\u5206\u6790\uff0c\u4ee5\u53ca\u8de8\u7269\u79cd\u529f\u80fd\u6ce8\u91ca\u6bd4\u5bf9\u3002\u5373\u4fbf\u5df2\u6709\u57fa\u56e0\u7ec4\u5e8f\u5217\uff0c\u8f6c\u5f55\u7ec4\u4ecd\u53ef\u7528\u4e8e\u4f18\u5316\u6ce8\u91ca\u3001\u8865\u5145\u4f4e\u8868\u8fbe\u6216\u7ec4\u7ec7\u7279\u5f02\u57fa\u56e0\uff0c\u63d0\u9ad8\u6bd4\u8f83\u5206\u6790\u7684\u51c6\u786e\u6027\u3002\u5728\u7cfb\u7edf\u53d1\u80b2\u7814\u7a76\u4e2d\uff0c\u8f6c\u5f55\u7ec4\u7ec4\u88c5\u4e3a\u7b5b\u9009\u76f4\u7cfb\u540c\u6e90\u57fa\u56e0\u548c\u6784\u5efa\u5927\u89c4\u6a21\u6838\u57fa\u56e0\u6570\u636e\u77e9\u9635\u63d0\u4f9b\u5173\u952e\u8d44\u6e90\u3002\u76f8\u8f83\u4e8e\u4f20\u7edf\u5c11\u6570\u5206\u5b50\u6807\u8bb0\uff0c\u57fa\u4e8e\u8f6c\u5f55\u7ec4\u7684\u591a\u57fa\u56e0\u7cfb\u7edf\u6811\u5177\u6709\u66f4\u9ad8\u7684\u5206\u8fa8\u7387\u4e0e\u7edf\u8ba1\u652f\u6301\u5ea6\uff0c\u540c\u65f6\u53ef\u7ed3\u5408\u9009\u62e9\u538b\u529b\u5206\u6790\u4e0e\u8868\u8fbe\u5206\u5316\u7814\u7a76\uff0c\u63ed\u793a\u9002\u5e94\u6027\u6f14\u5316\u673a\u5236\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6bf7322 elementor-widget elementor-widget-heading\" data-id=\"6bf7322\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Trinity<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-446b3ed elementor-widget elementor-widget-text-editor\" data-id=\"446b3ed\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u5728\u4f17\u591a de novo \u8f6c\u5f55\u7ec4\u7ec4\u88c5\u5de5\u5177\u4e2d\uff0c<strong data-start=\"21\" data-end=\"32\">Trinity<\/strong> \u662f\u5e94\u7528\u6700\u5e7f\u6cdb\u3001\u5f15\u7528\u7387\u6700\u9ad8\u7684\u7b97\u6cd5\u6846\u67b6\u4e4b\u4e00\u3002\u5176\u6838\u5fc3\u4f18\u52bf\u5728\u4e8e\u5c06\u590d\u6742\u7684\u8f6c\u5f55\u672c\u91cd\u5efa\u95ee\u9898\u62c6\u89e3\u4e3a\u4e09\u4e2a\u76f8\u4e92\u8854\u63a5\u4f46\u529f\u80fd\u72ec\u7acb\u7684\u6a21\u5757\uff1a<strong data-start=\"89\" data-end=\"123\">Inchworm\u3001Chrysalis \u548c Butterfly<\/strong>\uff0c\u5206\u522b\u8d1f\u8d23\u521d\u59cb contig \u6784\u5efa\u3001\u56fe\u7ed3\u6784\u5212\u5206\u4e0e\u6700\u7ec8\u8f6c\u5f55\u672c\u89e3\u6790\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e82f444 elementor-widget elementor-widget-heading\" data-id=\"e82f444\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">\u5b89\u88c5<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1ef8f48 elementor-widget elementor-widget-code-highlight\" data-id=\"1ef8f48\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>mamba install bioconda::trinity<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a457ea4 elementor-widget elementor-widget-heading\" data-id=\"a457ea4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u7ec4\u88c5<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1c34d63 elementor-widget elementor-widget-text-editor\" data-id=\"1c34d63\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u5728\u7ec4\u88c5\u4e4b\u524d\u8981\u5b8c\u6210\u6d4b\u5e8f\u6570\u636e\u7684\u6307\u63a7<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2081ba7 elementor-widget elementor-widget-code-highlight\" data-id=\"2081ba7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>fastp -i Unknown_CS440-004H0001_good_1.fq.gz  \\\n-I Unknown_CS440-004H0001_good_2.fq.gz \\\n-o Clean_Unknown_CS440-004H0001_good_1.fq.gz \\\n-O Clean_Unknown_CS440-004H0001_good_2.fq.gz\n-t 8 \\\n-f 15 \\\n-5<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-58169cc elementor-widget elementor-widget-text-editor\" data-id=\"58169cc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u968f\u540e\u8fdb\u884c\u5b8c\u5168\u65e0\u53c2\u7684de novo \u8f6c\u5f55\u7ec4\u7ec4\u88c5<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f183a16 elementor-widget elementor-widget-code-highlight\" data-id=\"f183a16\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>Trinity --seqType fq --max_memory 300G \\\n--left Ra_57_Pelophylax_nigromaculatus_1.fastq.gz \\\n--right Ra_57_Pelophylax_nigromaculatus_2.fastq.gz \\\n--CPU 20  --output Ra_57_Pelophylax_nigromaculatus_trinity\n\n# --seqType \u6570\u636e\u7684\u7c7b\u578b\n# --max_memory \u6700\u5927\u5185\u5b58\n# --left \u53cc\u7aef\u6d4b\u5e8f\u6570\u636e\n# --right \u53cc\u7aef\u6d4b\u5e8f\u6570\u636e\n# --CPU 20 \u8fd0\u884c\u7684\u7ebf\u7a0b\u6570\n# --output \u8f93\u51fa\u6587\u4ef6\u5939<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-70ce60c elementor-widget elementor-widget-text-editor\" data-id=\"70ce60c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Trinity \u8fd0\u884c\u5b8c\u6210\u540e\uff0c\u6700\u6838\u5fc3\u7684\u8f93\u51fa\u6587\u4ef6\u4e3a <code>Trinity.fasta<\/code>\uff0c\u5373\u7ec4\u88c5\u5f97\u5230\u7684\u5168\u90e8\u8f6c\u5f55\u672c\u5e8f\u5217\u96c6\u5408\u3002\u7136\u800c\uff0c\u9700\u8981\u660e\u786e\u7684\u662f\uff0c\u8fd9\u4e00\u6b65\u5e76\u4e0d\u610f\u5473\u7740\u5206\u6790\u6d41\u7a0b\u7684\u7ed3\u675f\u3002\u7531\u4e8e\u8f6c\u5f55\u7ec4\u7ec4\u88c5\u672c\u8eab\u4f1a\u4fdd\u7559\u53ef\u53d8\u526a\u63a5\u5f02\u6784\u4f53\uff08isoforms\uff09\u4ee5\u53ca\u9ad8\u5ea6\u76f8\u4f3c\u7684\u5197\u4f59\u5e8f\u5217\uff0c\u539f\u59cb\u7ec4\u88c5\u7ed3\u679c\u901a\u5e38\u5b58\u5728\u4e00\u5b9a\u7a0b\u5ea6\u7684\u5197\u4f59\u4e0e\u8f6c\u5f55\u672c\u91cd\u590d\u3002\u5982\u679c\u76f4\u63a5\u7528\u4e8e\u4e0b\u6e38\u5206\u6790\uff08\u5982\u529f\u80fd\u6ce8\u91ca\u3001\u540c\u6e90\u57fa\u56e0\u7b5b\u9009\u6216\u7cfb\u7edf\u53d1\u80b2\u6784\u5efa\uff09\uff0c\u53ef\u80fd\u4f1a\u5f15\u5165\u504f\u5dee\u6216\u653e\u5927\u7edf\u8ba1\u566a\u97f3\u3002<\/p><p>\u56e0\u6b64\uff0c\u5728\u6b63\u5f0f\u5f00\u5c55\u540e\u7eed\u5206\u6790\u4e4b\u524d\uff0c\u901a\u5e38\u9700\u8981\u8fdb\u884c\u53bb\u5197\u4f59\uff08redundancy removal\uff09\u5904\u7406\u3002\u56f4\u7ed5\u8fd9\u4e00\u73af\u8282\uff0c\u5b66\u754c\u5b58\u5728\u4e0d\u540c\u7b56\u7565\uff0c\u4e3b\u8981\u53ef\u5f52\u7eb3\u4e3a\u4e09\u7c7b\uff1a<br \/>1\uff09\u76f4\u63a5\u5bf9\u7ec4\u88c5\u5f97\u5230\u7684\u8f6c\u5f55\u672c\u5e8f\u5217\u8fdb\u884c\u805a\u7c7b\u53bb\u5197\u4f59\uff0c\u5e76\u5728\u6bcf\u4e2a cluster \u4e2d\u63d0\u53d6\u6700\u957f\u8f6c\u5f55\u672c\u4f5c\u4e3a\u4ee3\u8868\u5e8f\u5217\uff1b<br \/>2\uff09\u5bf9 TransDecoder \u9884\u6d4b\u5f97\u5230\u7684 CDS \u6216\u86cb\u767d\u5e8f\u5217\u8fdb\u884c\u76f8\u4f3c\u6027\u805a\u7c7b\u53bb\u5197\u4f59\uff1b<br \/>3\uff09\u5728 TransDecoder \u9884\u6d4b\u7ed3\u679c\u4e2d\uff0c\u4ec5\u4fdd\u7559\u6bcf\u4e2a\u57fa\u56e0\u5bf9\u5e94\u7684\u6700\u957f CDS \u6216\u86cb\u767d\u5e8f\u5217\u3002<\/p><p>\u603b\u4f53\u800c\u8a00\uff0c\u8fd9\u4e09\u79cd\u7b56\u7565\u5728\u5b8f\u89c2\u7edf\u8ba1\u7ed3\u679c\uff08\u5982\u57fa\u56e0\u6570\u76ee\u3001BUSCO \u5b8c\u6574\u5ea6\u6216\u7cfb\u7edf\u53d1\u80b2\u62d3\u6251\u7ed3\u6784\uff09\u4e0a\u5dee\u5f02\u901a\u5e38\u8f83\u5c0f\uff0c\u5bf9\u6574\u4f53\u7814\u7a76\u7ed3\u8bba\u5f71\u54cd\u6709\u9650\u3002\u56e0\u6b64\uff0c\u5728\u65b9\u6cd5\u9009\u62e9\u4e0a\u66f4\u5f3a\u8c03\u6d41\u7a0b\u7684\u4e00\u81f4\u6027\u4e0e\u53ef\u91cd\u590d\u6027\uff0c\u800c\u975e\u7edd\u5bf9\u4f18\u52a3\u3002<\/p><p>\u672c\u6587\u91c7\u7528\u7b2c\u4e00\u79cd\u7b56\u7565\uff0c\u5373\u76f4\u63a5\u57fa\u4e8e\u7ec4\u88c5\u5f97\u5230\u7684\u8f6c\u5f55\u672c\u5e8f\u5217\u8fdb\u884c\u76f8\u4f3c\u6027\u805a\u7c7b\u53bb\u5197\u4f59\uff0c\u5e76\u63d0\u53d6\u6bcf\u4e2a\u805a\u7c7b\u4e2d\u6700\u957f\u7684\u8f6c\u5f55\u672c\u4f5c\u4e3a\u4ee3\u8868\u5e8f\u5217\uff0c\u4ee5\u6784\u5efa\u540e\u7eed\u5206\u6790\u6240\u9700\u7684\u975e\u5197\u4f59\u8f6c\u5f55\u672c\u6570\u636e\u96c6\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-588a158 elementor-widget elementor-widget-heading\" data-id=\"588a158\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">\u4f7f\u7528Trinity\u81ea\u5e26\u7684\u811a\u672c\uff0c\u63d0\u53d6\u6700\u957f\u8f6c\u5f55\u672c<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-17f1a60 elementor-widget elementor-widget-code-highlight\" data-id=\"17f1a60\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>\n~\/miniforge3\/envs\/Trinity\/opt\/trinity-2.15.2\/util\/misc\/get_longest_isoform_seq_per_trinity_gene.pl Ra_59_Pelophylax_nigromaculatus_trinity.Trinity.fasta > Long_Ra_59_Pelophylax_nigromaculatus_trinity.Trinity.fasta<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cdc9703 elementor-widget elementor-widget-heading\" data-id=\"cdc9703\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">\u4f7f\u7528cd-hit\u53bb\u5197\u4f59<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5f835f5 elementor-widget elementor-widget-code-highlight\" data-id=\"5f835f5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>cd-hit-est \\\n-i Long_Ra_59_Pelophylax_nigromaculatus_trinity.Trinity.fasta \\\n-o cd_Long_Ra_59_Pelophylax_nigromaculatus_trinity.Trinity.fasta -T 20 -M 0 -c 0.99<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-534bf3b elementor-widget elementor-widget-heading\" data-id=\"534bf3b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title 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data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u9884\u6d4b\u5230\u7684CDS\u548cPEP\u5c31\u53ef\u4ee5\u7528\u4e8e\u7cfb\u7edf\u53d1\u80b2\u3001\u6bd4\u8f83\u57fa\u56e0\u7ec4\u548c\u6bd4\u8f83\u8f6c\u5f55\u7ec4\u7684\u4e0b\u6e38\u5206\u6790\u4e86\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>\u5f15\u8a00 \u968f\u7740 RNA-seq \u6280\u672f\u7684\u666e\u53ca\uff0c\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u5df2\u6210\u4e3a\u89e3\u6790\u57fa\u56e0\u8868\u8fbe\u4e0e\u529f\u80fd\u57fa\u56e0\u7ec4\u6210\u5206\u7684\u6838\u5fc3\u624b\u6bb5\u3002\u5bf9\u4e8e\u7f3a\u4e4f\u9ad8\u8d28\u91cf &#8230; <a title=\"\u57fa\u4e8eTrinity\u8fdb\u884c\u65e0\u53c2\u8f6c\u5f55\u7ec4\u7684\u7ec4\u88c5\" class=\"read-more\" href=\"http:\/\/www.majunpeng.com\/index.php\/2026\/02\/24\/%e5%9f%ba%e4%ba%8etrinity%e8%bf%9b%e8%a1%8c%e6%97%a0%e5%8f%82%e8%bd%ac%e5%bd%95%e7%bb%84%e7%9a%84%e7%bb%84%e8%a3%85\/\" aria-label=\"\u9605\u8bfb \u57fa\u4e8eTrinity\u8fdb\u884c\u65e0\u53c2\u8f6c\u5f55\u7ec4\u7684\u7ec4\u88c5\">\u9605\u8bfb\u66f4\u591a<\/a><\/p>\n","protected":false},"author":1,"featured_media":2937,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_crdt_document":"","footnotes":""},"categories":[9],"tags":[],"class_list":["post-2936","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-9"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>\u57fa\u4e8eTrinity\u8fdb\u884c\u65e0\u53c2\u8f6c\u5f55\u7ec4\u7684\u7ec4\u88c5 - \u5c0f\u9a6c\u751f\u4fe1\u65e5\u8bb0<\/title>\n<meta name=\"robots\" content=\"index, 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