{"id":2826,"date":"2026-01-23T18:39:57","date_gmt":"2026-01-23T10:39:57","guid":{"rendered":"http:\/\/www.majunpeng.com\/?p=2826"},"modified":"2026-01-23T23:43:02","modified_gmt":"2026-01-23T15:43:02","slug":"%e4%bd%bf%e7%94%a8fastqc%e3%80%81fastp%e5%92%8ctrimmomatic%e5%af%b9%e4%ba%8c%e4%bb%a3%e6%b5%8b%e5%ba%8f%e6%95%b0%e6%8d%ae%e8%bf%9b%e8%a1%8c%e8%b4%a8%e9%87%8f%e6%a3%80%e6%b5%8b%e6%98%af%e8%b4%a8","status":"publish","type":"post","link":"http:\/\/www.majunpeng.com\/index.php\/2026\/01\/23\/%e4%bd%bf%e7%94%a8fastqc%e3%80%81fastp%e5%92%8ctrimmomatic%e5%af%b9%e4%ba%8c%e4%bb%a3%e6%b5%8b%e5%ba%8f%e6%95%b0%e6%8d%ae%e8%bf%9b%e8%a1%8c%e8%b4%a8%e9%87%8f%e6%a3%80%e6%b5%8b%e6%98%af%e8%b4%a8\/","title":{"rendered":"\u4f7f\u7528Fastqc\u3001Fastp\u548cTrimmomatic\u5bf9\u4e8c\u4ee3\u6d4b\u5e8f\u6570\u636e\u8fdb\u884c\u8d28\u91cf\u68c0\u6d4b\u662f\u8d28\u63a7"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2826\" class=\"elementor elementor-2826\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-34a0ce0 e-flex e-con-boxed e-con e-parent\" data-id=\"34a0ce0\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-52c9a84 elementor-widget elementor-widget-heading\" data-id=\"52c9a84\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u5f15\u8a00<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-eba4f36 elementor-widget elementor-widget-text-editor\" data-id=\"eba4f36\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"text-base my-auto mx-auto pb-10 [--thread-content-margin:--spacing(4)] @w-sm\/main:[--thread-content-margin:--spacing(6)] @w-lg\/main:[--thread-content-margin:--spacing(16)] px-(--thread-content-margin)\"><div class=\"[--thread-content-max-width:40rem] @w-lg\/main:[--thread-content-max-width:48rem] mx-auto max-w-(--thread-content-max-width) flex-1 group\/turn-messages focus-visible:outline-hidden relative flex w-full min-w-0 flex-col agent-turn\" tabindex=\"-1\"><div class=\"flex max-w-full flex-col grow\"><div class=\"min-h-8 text-message relative flex w-full flex-col items-end gap-2 text-start break-words whitespace-normal [.text-message+&amp;]:mt-1\" dir=\"auto\" data-message-author-role=\"assistant\" data-message-id=\"b2c97e2b-e816-4d23-a5d7-cd98858f8d86\" data-message-model-slug=\"gpt-5-2\"><div class=\"flex w-full flex-col gap-1 empty:hidden first:pt-[1px]\"><div class=\"markdown prose dark:prose-invert w-full wrap-break-word light markdown-new-styling\"><p data-start=\"0\" data-end=\"140\">\u5728\u5f00\u5c55\u6bd4\u5bf9\u3001\u7ec4\u88c5\u6216\u53d8\u5f02\u68c0\u6d4b\u7b49\u4e0b\u6e38\u5206\u6790\u4e4b\u524d\uff0c<strong data-start=\"21\" data-end=\"49\">\u4e8c\u4ee3\u6d4b\u5e8f\u6570\u636e\u7684\u8d28\u91cf\u63a7\u5236\uff08QC\uff09\u662f\u4e0d\u53ef\u7701\u7565\u7684\u7b2c\u4e00\u6b65<\/strong>\u3002\u5c3d\u7ba1 Illumina \u7b49\u4e8c\u4ee3\u6d4b\u5e8f\u5e73\u53f0\u5177\u6709\u9ad8\u901a\u91cf\u548c\u9ad8\u6027\u4ef7\u6bd4\u4f18\u52bf\uff0c\u4f46\u5176\u6d4b\u5e8f\u8fc7\u7a0b\u4ecd\u4f1a\u5f15\u5165\u591a\u79cd\u6280\u672f\u566a\u97f3\uff0c\u5982 reads 3\u2032 \u7aef\u78b1\u57fa\u8d28\u91cf\u4e0b\u964d\u3001\u63a5\u5934\u6c61\u67d3\u3001PCR \u6269\u589e\u504f\u597d\u53ca\u968f\u673a\u6d4b\u5e8f\u9519\u8bef\u7b49\u3002\u8fd9\u4e9b\u4f4e\u8d28\u91cf\u5e8f\u5217\u82e5\u76f4\u63a5\u8fdb\u5165\u4e0b\u6e38\u5206\u6790\uff0c\u5c06\u663e\u8457\u5f71\u54cd\u7ed3\u679c\u7684\u53ef\u9760\u6027\uff1a\u5728\u53c2\u8003\u57fa\u56e0\u7ec4\u6bd4\u5bf9\u4e2d\u4f1a\u589e\u52a0\u9519\u914d\u4e0e\u591a\u91cd\u6bd4\u5bf9\uff0c\u5728\u53d8\u5f02\u68c0\u6d4b\u4e2d\u4f1a\u5f15\u5165\u5047\u9633\u6027\u4fe1\u53f7\uff0c\u5728 de novo \u7ec4\u88c5\u4e2d\u5219\u53ef\u80fd\u5bfc\u81f4 contig \u65ad\u88c2\u6216\u9519\u8bef\u62fc\u63a5\u3002\u56e0\u6b64\uff0c\u8d28\u63a7\u7684\u76ee\u7684\u5e76\u975e\u201c\u7f8e\u5316\u6570\u636e\u201d\uff0c\u800c\u662f<strong data-start=\"259\" data-end=\"284\">\u6700\u5927\u9650\u5ea6\u964d\u4f4e\u6280\u672f\u8bef\u5dee\uff0c\u4fdd\u7559\u771f\u5b9e\u7684\u751f\u7269\u5b66\u4fe1\u606f<\/strong>\u3002\u57fa\u4e8e\u6b64\uff0cFastQC\u3001Fastp \u548c Trimmomatic \u7b49\u5de5\u5177\u88ab\u5e7f\u6cdb\u7528\u4e8e\u4e8c\u4ee3\u6d4b\u5e8f\u6570\u636e\u7684\u8d28\u91cf\u8bc4\u4f30\u4e0e\u8fc7\u6ee4\uff0c\u4ece\u4e0d\u540c\u5c42\u9762\u6784\u5efa\u8d77\u7a33\u5b9a\u3001\u53ef\u9760\u7684\u4e8c\u4ee3\u6d4b\u5e8f\u6570\u636e\u8d28\u63a7\u6d41\u7a0b\uff0c\u4e3a\u540e\u7eed\u5206\u6790\u5960\u5b9a\u575a\u5b9e\u57fa\u7840\u3002<\/p><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0c13761 elementor-widget elementor-widget-heading\" data-id=\"0c13761\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Fastqc<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ac239c9 elementor-widget elementor-widget-heading\" data-id=\"ac239c9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">\u5b89\u88c5<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-53a16b3 elementor-widget elementor-widget-code-highlight\" data-id=\"53a16b3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-javascript line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-javascript\">\n\t\t\t\t\t<xmp>mamba install bioconda::fastqc<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cf2b93a elementor-widget elementor-widget-heading\" data-id=\"cf2b93a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u8d28\u63a7<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-516e159 elementor-widget elementor-widget-code-highlight\" data-id=\"516e159\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-javascript line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-javascript\">\n\t\t\t\t\t<xmp>fastqc Clean_P636_YMiao_A01v1_S0-1_S1_L002_R1_001.fastq.gz \\\nClean_P636_YMiao_A01v1_S0-1_S1_L002_R2_001.fastq.gz<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fcc1254 elementor-widget elementor-widget-text-editor\" data-id=\"fcc1254\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u6bcf\u4e2a\u6d4b\u5e8f\u6587\u4ef6\u4f1a\u8f93\u51fa\u4e00\u4e2a\u5bf9\u5e94\u7684html\u6587\u4ef6\uff0c\u8bb0\u5f55\u4e86\u6bcf\u4e2a\u6307\u6807\u7684\u68c0\u6d4b\u60c5\u51b5\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-af8430a elementor-widget elementor-widget-heading\" data-id=\"af8430a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">\u7ed3\u679c\u89e3\u8bfb<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-06d1179 elementor-widget elementor-widget-text-editor\" data-id=\"06d1179\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u901a\u8fc7\u4e0b\u521711\u9879\u6307\u6807\u6765\u8fdb\u884c\u6d4b\u5e8f\u8d28\u91cf\u7684\u8bc4\u4f30\uff1a<\/p><p>1. Basic Statistics<\/p><p>\u7edf\u8ba1\u6570\u636e\u91cf\u548creads\u957f\u5ea6\u7b49\u57fa\u672c\u6307\u6807<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-986298b elementor-widget elementor-widget-image\" data-id=\"986298b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"592\" height=\"276\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/ScreenShot_2026-01-23_193401_844.png\" class=\"attachment-large size-large wp-image-2848\" alt=\"\" srcset=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/ScreenShot_2026-01-23_193401_844.png 592w, http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/ScreenShot_2026-01-23_193401_844-300x140.png 300w\" sizes=\"(max-width: 592px) 100vw, 592px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-56a4895 elementor-widget elementor-widget-text-editor\" data-id=\"56a4895\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>2. Per base sequence quality<\/p><p>\u7528\u7bb1\u5f0f\u56fe\u7684\u65b9\u5f0f\u5c55\u793a\u6570\u636e\u8d28\u91cf\uff0c\u56fe\u4e2dX\u8f74\u6bcf1\u4e2a\u4f4d\u7f6e\uff0c\u90fd\u662f\u8be5\u4f4d\u7f6e\u7684\u6240\u6709\u5e8f\u5217\u7684\u6d4b\u5e8f\u8d28\u91cf\u7684\u7edf\u8ba1\u3002\u7eb5\u8f74\u662f\u8d28\u91cf\u5f97\u5206\uff0cQ =-10*log10(p)\uff0cp\u4e3a\u6d4b\u9519\u7684\u6982\u7387\u3002\u6240\u4ee5\u4e00\u6761reads\u67d0\u4f4d\u7f6e\u51fa\u9519\u6982\u73870.01\u65f6\uff0c\u5176quality\u5c31\u662f20\u3002\u6a2a\u8f74\u662f\u6d4b\u5e8f\u5e8f\u5217\u7684\u4f4d\u7f6e\u3002\u84dd\u8272\u7ebf\u662f\u5404\u4e2a\u4f4d\u7f6e\u7684\u5e73\u5747\u503c\u7684\u8fde\u7ebf\u3002\u4e00\u822c\u8981\u6c42\u6b64\u56fe\u4e2d\uff0c\u6240\u6709\u4f4d\u7f6e\u768410%\u5206\u4f4d\u6570\u5927\u4e8e20,\u4e5f\u5c31\u662f\u5e38\u8bf4\u7684Q20\u8fc7\u6ee4\u3002\u3002\u5982\u679c\u4efb\u4f55\u78b1\u57fa\u8d28\u91cf\u4f4e\u4e8e10,\u6216\u8005\u662f\u4efb\u4f55\u4e2d\u4f4d\u6570\u4f4e\u4e8e25\u62a5\u8b66\uff0c\u5982\u679c\u4efb\u4f55\u78b1\u57fa\u8d28\u91cf\u4f4e\u4e8e5,\u6216\u8005\u662f\u4efb\u4f55\u4e2d\u4f4d\u6570\u4f4e\u4e8e20\u62a5\u9519\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-25c4396 elementor-widget elementor-widget-image\" data-id=\"25c4396\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"800\" height=\"600\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d.png\" class=\"attachment-large size-large wp-image-2829\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b7e00a7 elementor-widget elementor-widget-text-editor\" data-id=\"b7e00a7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>3. Per tile sequence quality<\/p><p>\u8fd9\u4e00\u6a21\u5757\u662f\u68c0\u67e5\u5728\u6d4b\u5e8f\u5e73\u53f0\u4e0a\uff0creads\u4e2d\u6bcf\u4e00\u4e2a\u78b1\u57fa\u4f4d\u7f6e\u5728\u4e0d\u540c\u7684\u6d4b\u5e8f\u5c0f\u5b54\u4e4b\u95f4\u7684\u504f\u79bb\u5ea6\uff0c\u504f\u79bb\u5ea6\u8d8a\u9ad8\uff0c\u78b1\u57fa\u8d28\u91cf\u8d8a\u5dee\u3002\u7eb5\u8f74\u8868\u793a\u6d4b\u5e8f\u5c0f\u5b54\uff0c\u84dd\u8272\u8868\u793a\u4f4e\u4e8e\u5e73\u5747\u504f\u79bb\u5ea6\uff0c\u8d8a\u7ea2\u5219\u8bf4\u660e\u504f\u79bb\u5e73\u5747\u8d28\u91cf\u65b9\u5dee\u8d8a\u591a\uff0c\u4e5f\u5c31\u662f\u8bf4\u8d28\u91cf\u8d8a\u5dee\uff0c\u672c\u56fe\u4e2d\u90fd\u662f\u84dd\u8272\u8868\u660e\u8d28\u91cf\u5f88\u597d\u3002\u5982\u679c\u51fa\u73b0\u8d28\u91cf\u95ee\u9898\u53ef\u80fd\u662f\u77ed\u6682\u7684\uff0c\u5982\u6709\u6c14\u6ce1\u4ea7\u751f\uff0c\u4e5f\u53ef\u80fd\u662f\u957f\u671f\u7684\uff0c\u5982\u5728\u67d0\u4e00\u5c0f\u5b54\u4e2d\u5b58\u5728\u6742\u8d28\u3002\u504f\u79bb\u5ea6\u5c0f\u4e8e\u5e73\u5747\u503c2\u4ee5\u4e0a\u62a5\u8b66\uff0c\u504f\u79bb\u5ea6\u5c0f\u4e8e\u5e73\u5747\u503c5\u4ee5\u4e0a\u4e0d\u5408\u683c\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-95d4393 elementor-widget elementor-widget-image\" data-id=\"95d4393\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"800\" height=\"600\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-1.png\" class=\"attachment-large size-large wp-image-2836\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8178c04 elementor-widget elementor-widget-text-editor\" data-id=\"8178c04\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>4. Per sequence quality scores<\/p><p>\u8fd9\u662f\u4e3a\u4e86\u68c0\u6d4b\u4e00\u90e8\u5206\u8d28\u91cf\u7279\u522b\u5dee\u7684reads\uff0c\u5982\u679c\u6709\u5219\u4f1a\u5728\u56fe\u4e0a\u51fa\u73b0\u591a\u4e2a\u5cf0\uff0c\u5982\u5728\u6d4b\u5e8f\u4eea\u8fb9\u7f18\u7684reads\u3002\u7eb5\u8f74\u662freads\u6570\u76ee\uff0c\u6a2a\u8f74\u662f\u8d28\u91cf\u5206\u6570\uff0c\u4ee3\u8868\u4e0d\u540cPhred\u503c\u5bf9\u5e94\u4e86\u591a\u5c11\u7684reads\u3002\u672c\u56fe\u4e2d\uff0c\u6d4b\u5e8f\u7ed3\u679c\u4e3b\u8981\u96c6\u4e2d\u5728\u9ad8\u5206\u4e2d\uff0c\u8bc1\u660e\u6d4b\u5e8f\u8d28\u91cf\u826f\u597d\u3002\u5f53\u5cf0\u503c\u5c0f\u4e8e27\uff08\u9519\u8bef\u73870.2%\uff09\u65f6\u8b66\u62a5\uff0c\u5f53\u5cf0\u503c\u5c0f\u4e8e20\uff08\u9519\u8bef\u73871%\uff09\u65f6\u4e0d\u5408\u683c\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-70d1a01 elementor-widget elementor-widget-image\" data-id=\"70d1a01\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"600\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-2.png\" class=\"attachment-large size-large wp-image-2837\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7353846 elementor-widget elementor-widget-text-editor\" data-id=\"7353846\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>5. Per base sequence content<\/p><p>\u5c55\u793a\u78b1\u57fa\u542b\u91cf\u5206\u5e03\uff0c\u5b83\u6839\u636e\u78b1\u57fa\u7684\u4f4d\u7f6e\u5bf9\u6bcf\u4e2a\u4f4d\u7f6e\u4e0a\u7684A,C,G,T\u7684\u542b\u91cf\u8fdb\u884c\u7edf\u8ba1\uff0c\u6a2a\u8f74\u4e3a\u4f4d\u7f6e\uff0c\u7eb5\u8f74\u4e3a\u767e\u5206\u6bd4\u3002\u6b63\u5e38\u60c5\u51b5\u4e0b\u56db\u79cd\u78b1\u57fa\u7684\u51fa\u73b0\u9891\u7387\u5e94\u8be5\u662f\u63a5\u8fd1\u7684\uff0c\u800c\u4e14\u6ca1\u6709\u4f4d\u7f6e\u5dee\u5f02\u3002\u56e0\u6b64\u597d\u7684\u6837\u672c\u4e2d\u56db\u6761\u7ebf\u5e94\u8be5\u5e73\u884c\u4e14\u63a5\u8fd1\u3002\u5f53\u90e8\u5206\u4f4d\u7f6e\u78b1\u57fa\u7684\u6bd4\u4f8b\u51fa\u73b0bias\u65f6\uff0c\u5373\u56db\u6761\u7ebf\u5728\u67d0\u4e9b\u4f4d\u7f6e\u7eb7\u4e71\u4ea4\u7ec7\uff0c\u5f80\u5f80\u63d0\u793a\u6211\u4eec\u6709overrepresented sequence\u7684\u6c61\u67d3\u3002\u5f53\u6240\u6709\u4f4d\u7f6e\u7684\u78b1\u57fa\u6bd4\u4f8b\u4e00\u81f4\u7684\u8868\u73b0\u51fabias\u65f6\uff0c\u5373\u56db\u6761\u7ebf\u5e73\u884c\u4f46\u5206\u5f00\uff0c\u5f80\u5f80\u4ee3\u8868\u6587\u5e93\u6709bias (\u5efa\u5e93\u8fc7\u7a0b\u6216\u672c\u8eab\u7279\u70b9)\uff0c\u6216\u8005\u662f\u6d4b\u5e8f\u4e2d\u7684\u7cfb\u7edf\u8bef\u5dee\u3002\u5f53\u4efb\u4e00\u4f4d\u7f6e\u7684A\/T\u6bd4\u4f8b\u4e0eG\/C\u6bd4\u4f8b\u76f8\u5dee\u8d85\u8fc710%\u53d1\u51fa\u8b66\u62a5\uff0c\u8d85\u8fc720%\u5219\u6570\u636e\u4e0d\u5408\u683c\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a305b93 elementor-widget elementor-widget-image\" data-id=\"a305b93\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"600\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-3.png\" class=\"attachment-large size-large wp-image-2838\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-63caaf6 elementor-widget elementor-widget-text-editor\" data-id=\"63caaf6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>6. Per sequence GC content<\/p><p>\u7ea2\u8272\u66f2\u7ebf\u662f\u5b9e\u9645\u7684\u6d4b\u5e8fGC\u542b\u91cf\u5206\u5e03\u56fe\uff0c\u800c\u84dd\u8272\u66f2\u7ebf\u5219\u662f\u7406\u8bba\u5206\u5e03\uff08\u6b63\u6001\u5206\u5e03\uff0c\u4e0d\u8fc7\u5747\u503c\u4e0d\u4e00\u5b9a\u90fd\u662f50%\uff0c\u800c\u662f\u7531\u5e73\u5747GC\u542b\u91cf\u63a8\u65ad\u7684\uff09\u3002\u5982\u679c\u7ea2\u8272\u66f2\u7ebf\u5f62\u72b6\u5b58\u5728\u6bd4\u8f83\u5927\u7684\u504f\u5dee\uff0c\u5f80\u5f80\u662f\u7531\u4e8e\u6587\u5e93\u6c61\u67d3\u9020\u6210\u7684\u3002\u7ea2\u8272\u66f2\u7ebf\u8d8a\u5e73\u6ed1\u8d8a\u597d\uff0c\u8d8a\u63a5\u8fd1\u84dd\u8272\u66f2\u7ebf\u8d8a\u597d\u3002\u5f62\u72b6\u63a5\u8fd1\u6b63\u6001\u4f46\u504f\u79bb\u7406\u8bba\u5206\u5e03\u7684\u60c5\u51b5\u63d0\u793a\u6211\u4eec\u53ef\u80fd\u6709\u7cfb\u7edf\u504f\u5dee\u3002\u504f\u79bb\u7406\u8bba\u5206\u5e03\u7684reads\u8d85\u8fc715%\u65f6\u53d1\u51fa\u8b66\u62a5\uff0c\u8d85\u8fc730%\u65f6\u62a5\u4e0d\u5408\u683c\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9c8afab elementor-widget elementor-widget-image\" data-id=\"9c8afab\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"600\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-4.png\" class=\"attachment-large size-large wp-image-2840\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6e7a918 elementor-widget elementor-widget-text-editor\" data-id=\"6e7a918\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>7. Per base N content<\/p><p>\u7eb5\u8f74\u662f\u767e\u5206\u542b\u91cf\uff0c\u6a2a\u8f74\u662fread\u7684\u4f4d\u7f6e\uff0c\u5f53\u6d4b\u5e8f\u4eea\u4e0d\u80fd\u786e\u5207\u5730\u6d4b\u5b9a\u51fa\u67d0\u4e00\u4e2a\u78b1\u57fa\u65f6\u5c31\u4f1a\u6807\u6ce8\u4e3aN\uff0c\u6b63\u5e38\u60c5\u51b5\u4e0bN\u7684\u6bd4\u4f8b\u662f\u5f88\u5c0f\u7684\uff0c\u6240\u4ee5\u56fe\u4e0a\u5e38\u5e38\u770b\u5230\u4e00\u6761\u76f4\u7ebf\u3002\u5f53\u770b\u5230\u6709\u5cf0\u65f6\uff0c\u8bf4\u660e\u6d4b\u5e8f\u51fa\u4e86\u95ee\u9898\u3002\u5f53\u4efb\u610f\u4f4d\u7f6e\u7684N\u7684\u6bd4\u4f8b\u8d85\u8fc75%\u8b66\u62a5\u8d85\u8fc720%\u4e0d\u5408\u683c\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-99c75c0 elementor-widget elementor-widget-image\" data-id=\"99c75c0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"600\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-5.png\" class=\"attachment-large size-large wp-image-2841\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-057379a elementor-widget elementor-widget-text-editor\" data-id=\"057379a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>8. Sequence Length Distribution<\/p><p>\u6bcf\u6b21\u6d4b\u5e8f\u4eea\u6d4b\u51fa\u6765\u7684\u957f\u5ea6\u5728\u7406\u8bba\u4e0a\u5e94\u8be5\u662f\u5b8c\u5168\u76f8\u7b49\u7684\uff0c\u4f46\u662f\u603b\u4f1a\u6709\u4e00\u4e9b\u504f\u5dee\uff0c\u5982\u6b64\u56fe\u4e2d\uff0c151bp\u662f\u4e3b\u8981\u7684\uff0c\u4f46\u662f\u8fd8\u662f\u6709\u5c11\u91cf\u7684150\u548c152bp\u7684\u957f\u5ea6\uff0c\u4e0d\u8fc7\u6570\u91cf\u6bd4\u8f83\u5c11\uff0c\u4e0d\u5f71\u54cd\u540e\u7eed\u5206\u6790\uff0c\u5f53\u6d4b\u5e8f\u7684\u957f\u5ea6\u6709\u5f88\u5927\u4e0d\u540c\u65f6\uff0c\u5219\u8868\u660e\u6d4b\u5e8f\u4eea\u5728\u6b64\u6b21\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\u4ea7\u751f\u7684\u6570\u636e\u4e0d\u53ef\u4fe1\uff0c\u4f46\u5bf9\u4e8e\u67d0\u4e9b\u6d4b\u5e8f\u5e73\u53f0\uff0c\u5177\u6709\u4e0d\u540c\u7684read\u957f\u5ea6\u662f\u5b8c\u5168\u6b63\u5e38\u7684\u3002\u5f53reads\u957f\u5ea6\u4e0d\u4e00\u81f4\u65f6\u8b66\u544a\uff0c\u5f53\u6709\u957f\u5ea6\u4e3a0\u7684read\u65f6\u4e0d\u5408\u683c\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-11afdf9 elementor-widget elementor-widget-image\" data-id=\"11afdf9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"600\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-6.png\" class=\"attachment-large size-large wp-image-2842\" alt=\"\" srcset=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-6.png 800w, http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-6-300x225.png 300w, http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-6-768x576.png 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7f8566e elementor-widget elementor-widget-text-editor\" data-id=\"7f8566e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>9. Sequence Duplication Levels<\/p><p>\u6a2a\u8f74\u4e3areads\u91cd\u590d\u7684\u6b21\u6570\uff0c\u7eb5\u8f74\u4e3a\u91cd\u590d\u6b21\u6570\u5bf9\u5e94\u7684reads\u5360\u4e0d\u91cd\u590d\u7684reads\u7684\u6bd4\u4f8b\u3002\u6d4b\u5e8f\u6df1\u5ea6\u8d8a\u9ad8\uff0c\u8d8a\u5bb9\u6613\u4ea7\u751f\u4e00\u5b9a\u7a0b\u5ea6\u7684duplication\uff0c\u8fd9\u662f\u6b63\u5e38\u7684\u73b0\u8c61\uff0c\u4f46\u5982\u679cduplication\u7684\u7a0b\u5ea6\u5f88\u9ad8\uff0c\u90a3\u4e48\u8868\u660e\u5b58\u5728\u5bcc\u96c6\u7684\u504f\u597d\uff08enrichment bias\uff09\uff08\u6bd4\u5982\uff1a\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\u7684PCR\u91cd\u590d\uff0c\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u4e2d\u67d0\u4e9b\u57fa\u56e0\u8868\u8fbe\u91cf\u9ad8\uff09\uff0c\u5e8f\u5217\u91cd\u590d\u6bd4\u4f8b\u8d8a\u9ad8\uff0c\u5219\u8868\u660e\u5b9e\u9645\u6709\u7528\u7684\u5e8f\u5217\u8d8a\u5c11\u3002\u56fe\u4e2d\u6709\u84dd\u7ea2\u4e24\u6761\u7ebf\uff0c\u84dd\u8272\u7ebf\u8868\u793a\u7684\u662f\u6587\u4ef6\u4e2d\u6240\u6709\u7684\u5e8f\u5217\u4e2dduplicate\u7a0b\u5ea6\u7684\u5206\u5e03\uff0c\u7ea2\u8272\u7ebf\u8868\u793a\u7684\u662f\u53bb\u5197\u4f59\u4e4b\u540e\u7684\u5e8f\u5217\uff0c\u542b\u91cf\u8868\u793a\u7684\u5728\u5168\u90e8\u5e8f\u5217\u90fd\u8003\u8651\u65f6\u4e0d\u540c\u5197\u4f59\u7a0b\u5ea6\u7684\u5e8f\u5217\u6240\u5360\u7684\u6bd4\u4f8b\u3002\u91cd\u590dreads\u5360\u603b\u6570\u7684\u6bd4\u4f8b\u5927\u4e8e20%\u65f6\u8b66\u62a5\uff0c\u5927\u4e8e50%\u65f6\u4e0d\u5408\u683c\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d4e0612 elementor-widget elementor-widget-image\" data-id=\"d4e0612\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"600\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-7.png\" class=\"attachment-large size-large wp-image-2843\" alt=\"\" srcset=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-7.png 800w, http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-7-300x225.png 300w, http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-7-768x576.png 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-48bd049 elementor-widget elementor-widget-text-editor\" data-id=\"48bd049\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>10. Overrepresented sequences<\/p><p>\u5982\u679c\u6709\u67d0\u4e2a\u5e8f\u5217\u5927\u91cf\u51fa\u73b0\uff0c\u5c31\u53eb\u505aover-represented\u3002\u6807\u51c6\u662f\u5360\u5168\u90e8reads\u76840.1%\u4ee5\u4e0a\u3002\u4f46\u662f\u56e0\u4e3a\u7528\u7684\u662fDuplicate sequences\u524d200,000\u6761\u6570\u636e\uff0c\u6240\u4ee5\u6709\u53ef\u80fdover-represented reads\u4e0d\u5728\u91cc\u9762\uff0c\u53c2\u8003\u610f\u4e49\u4e0d\u5927\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-941ad7f elementor-widget elementor-widget-text-editor\" data-id=\"941ad7f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>11. Adapter Content<\/p><p>\u6b64\u56fe\u8861\u91cf\u7684\u662f\u5e8f\u5217\u4e2d\u4e24\u7aefadapter\u7684\u60c5\u51b5\uff0c\u5982\u679c\u5728fastqc\u5206\u6790\u7684\u65f6\u5019-a\uff08\u6307\u5b9a\u542badapters\u5e8f\u5217\u6587\u4ef6\uff09\u9009\u9879\u6ca1\u6709\u5185\u5bb9\uff0c\u5219\u9ed8\u8ba4\u4f7f\u7528\u56fe\u4f8b\u4e2d\u7684\u901a\u7528adapter\u5e8f\u5217\u8fdb\u884c\u7edf\u8ba1\u3002\u542b\u6709adapter\u8d85\u8fc7\u6240\u6709reads\u76845%\u7684\u8b66\u544a\uff0c\u8d85\u8fc710%\u4e0d\u5408\u683c\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4e44424 elementor-widget elementor-widget-image\" data-id=\"4e44424\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"600\" src=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-8.png\" class=\"attachment-large size-large wp-image-2844\" alt=\"\" srcset=\"http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-8.png 800w, http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-8-300x225.png 300w, http:\/\/www.majunpeng.com\/wp-content\/uploads\/2026\/01\/\u4e0b\u8f7d-8-768x576.png 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8f4d5d3 elementor-widget elementor-widget-text-editor\" data-id=\"8f4d5d3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u4ee5\u6211\u8fd9\u4e2a\u6d4b\u5e8f\u6570\u636e\u4e3a\u4f8b\uff0c\u4e3b\u8981\u7684\u95ee\u9898\u662f\u542b\u6709\u5927\u91cf\u7684\u63a5\u5934\u5e8f\u5217\uff0c\u5e76\u4e14reads\u7684\u524d1-15\u4f4d\u7f6e\u7684\u78b1\u57fa\u4e0d\u662f\u5f88\u7a33\u5b9a\uff0c\u540e\u9762\u8981\u7740\u91cd\u5904\u7406\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c89ae4a elementor-widget elementor-widget-heading\" data-id=\"c89ae4a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Fastp\u5bf9\u539f\u59cb\u6570\u636e\u8fdb\u884c\u8fc7\u6ee4<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e8d3f55 elementor-widget elementor-widget-text-editor\" data-id=\"e8d3f55\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u5f53\u6211\u4eec\u901a\u8fc7FastQC\u5bf9\u6d4b\u5e8f\u6570\u636e\u8d28\u68c0\uff0c\u77e5\u9053\u6570\u636e\u6709\u54ea\u4e9b\u9519\u8bef\u540e\uff0c\u5c31\u53ef\u4ee5\u901a\u8fc7fastp\u548ctrimmomatic\u7b49\u8fc7\u6ee4\u8f6f\u4ef6\u8fdb\u884c\u9488\u5bf9\u6027\u7684\u8d28\u63a7\uff0c\u8fd9\u91cc\u6211\u4eec\u9009\u62e9fastp\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a8fbcaf elementor-widget elementor-widget-heading\" data-id=\"a8fbcaf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">\u5b89\u88c5<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c7d954a elementor-widget elementor-widget-code-highlight\" data-id=\"c7d954a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-javascript line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-javascript\">\n\t\t\t\t\t<xmp>mamba install bioconda::fastp<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5508f5c elementor-widget elementor-widget-heading\" data-id=\"5508f5c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">\u8fc7\u6ee4<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ebe8dd5 elementor-widget elementor-widget-code-highlight\" data-id=\"ebe8dd5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-javascript line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-javascript\">\n\t\t\t\t\t<xmp>fastp -i fastp_P636_YMiao_A01v1_S0-1_S1_L002_R1_001_fastqc.html \\\n-I fastp_P636_YMiao_A01v1_S0-1_S1_L002_R2_001_fastqc.html \\\n-o fastp_fastp_P636_YMiao_A01v1_S0-1_S1_L002_R1_001_fastqc.html \\\n-O fastp_fastp_P636_YMiao_A01v1_S0-1_S1_L002_R2_001_fastqc.html \\\n-t 8 \\\n-f 15 \\\n-5\n\n# -i,-i \u8f93\u5165\u7684\u6d4b\u5e8f\u6570\u636e\n# -o,-O \u8f93\u51fa\u7684\u8fc7\u6ee4\u540e\n\u6d4b\u5e8f\u6570\u636e\n# -t cpu\n# -f \u5220\u9664reads\u5934\u90e8\u524d15\u4e2a\u78b1\u57fa<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-ae46b06 e-flex e-con-boxed e-con e-parent\" data-id=\"ae46b06\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>\u5f15\u8a00 \u5728\u5f00\u5c55\u6bd4\u5bf9\u3001\u7ec4\u88c5\u6216\u53d8\u5f02\u68c0\u6d4b\u7b49\u4e0b\u6e38\u5206\u6790\u4e4b\u524d\uff0c\u4e8c\u4ee3\u6d4b\u5e8f\u6570\u636e\u7684\u8d28\u91cf\u63a7\u5236\uff08QC\uff09\u662f\u4e0d\u53ef\u7701\u7565\u7684\u7b2c\u4e00\u6b65\u3002\u5c3d\u7ba1 Ill &#8230; <a title=\"\u4f7f\u7528Fastqc\u3001Fastp\u548cTrimmomatic\u5bf9\u4e8c\u4ee3\u6d4b\u5e8f\u6570\u636e\u8fdb\u884c\u8d28\u91cf\u68c0\u6d4b\u662f\u8d28\u63a7\" class=\"read-more\" href=\"http:\/\/www.majunpeng.com\/index.php\/2026\/01\/23\/%e4%bd%bf%e7%94%a8fastqc%e3%80%81fastp%e5%92%8ctrimmomatic%e5%af%b9%e4%ba%8c%e4%bb%a3%e6%b5%8b%e5%ba%8f%e6%95%b0%e6%8d%ae%e8%bf%9b%e8%a1%8c%e8%b4%a8%e9%87%8f%e6%a3%80%e6%b5%8b%e6%98%af%e8%b4%a8\/\" aria-label=\"\u9605\u8bfb 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