{"id":2545,"date":"2025-12-10T20:26:15","date_gmt":"2025-12-10T12:26:15","guid":{"rendered":"http:\/\/www.majunpeng.com\/?p=2545"},"modified":"2025-12-15T11:27:30","modified_gmt":"2025-12-15T03:27:30","slug":"%e4%bd%bf%e7%94%a8%e5%85%a8%e9%95%bf%e8%bd%ac%e5%bd%95%e7%bb%84%e8%bf%9b%e8%a1%8c%e5%9f%ba%e5%9b%a0%e7%bb%84%e7%bb%93%e6%9e%84%e6%b3%a8%e9%87%8a","status":"publish","type":"post","link":"http:\/\/www.majunpeng.com\/index.php\/2025\/12\/10\/%e4%bd%bf%e7%94%a8%e5%85%a8%e9%95%bf%e8%bd%ac%e5%bd%95%e7%bb%84%e8%bf%9b%e8%a1%8c%e5%9f%ba%e5%9b%a0%e7%bb%84%e7%bb%93%e6%9e%84%e6%b3%a8%e9%87%8a\/","title":{"rendered":"\u4f7f\u7528\u5168\u957f\u8f6c\u5f55\u7ec4\u8fdb\u884c\u57fa\u56e0\u7ec4\u7ed3\u6784\u6ce8\u91ca"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2545\" class=\"elementor elementor-2545\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c067b4d e-flex e-con-boxed e-con e-parent\" data-id=\"c067b4d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8032250 elementor-widget elementor-widget-heading\" data-id=\"8032250\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">1. \u524d\u8a00<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dbf6615 elementor-widget elementor-widget-text-editor\" data-id=\"dbf6615\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u53bb\u5e74\u5e74\u521d\uff0c\u9996\u6b21\u5b8c\u6210\u4e86\u7b2c\u4e00\u4e2a\u7ec4\u88c5\u7684\u57fa\u56e0\u7ec4\u7684\u7ed3\u6784\u6ce8\u91ca\uff0c\u5e76\u4e14\u628a\u6d41\u7a0b\u8bb0\u5f55\u4e0b\u6765\u5199\u6210\u4e86\u535a\u5ba2\uff0c\u7f8e\u4e2d\u4e0d\u8db3\u662f\u57fa\u4e8e\u4e8c\u4ee3\u7684\u8f6c\u5f55\u7ec4\u5b8c\u6210\u7684\uff0c\u6240\u4ee5\u4eca\u5929\u6765\u8bb0\u5f55\u4e00\u6b21\u57fa\u4e8e\u4e8c\u4ee3\u548c\u4e09\u4ee3\u7684RNA-seq\u6570\u636e\u8fdb\u884c\u7ed3\u6784\u6ce8\u91ca\u7684\u8fc7\u7a0b\u3002\u53e6\u5916\uff0c\u672c\u6b21\u7684denovo\u9884\u6d4b\u4eceAgustus\u6362\u6210\u4e86ANNEVO\u3002\u8fd9\u4e2a\u535a\u5ba2\u66f4\u591a\u7684\u662f\u4e00\u4e2a\u6d41\u7a0b\uff0c\u4e00\u4e9b\u7ec6\u8282\u4e0d\u4f1a\u5f88\u8be6\u7ec6\uff0c\u5982\u679c\u770b\u4e0d\u61c2\uff0c\u53ef\u4ee5\u5148\u770b\u4e4b\u524d\u5173\u4e8e\u57fa\u56e0\u7ed3\u6784\u6ce8\u91ca\u7684\u535a\u5ba2\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-87d1707 elementor-widget elementor-widget-heading\" data-id=\"87d1707\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">2. \u8f6f\u4ef6\u4e0b\u8f7d<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-58debd6 elementor-widget elementor-widget-code-highlight\" data-id=\"58debd6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>git clone https:\/\/github.com\/xjtu-omics\/ANNEVO.git\r\ncd ANNEVO\r\nmmaba env create -f ANNEVO.yml -n Anno\r\nmamba activate Anno\r\nmamba create -n Gene_Anno\r\nmamba install bioconda::hisat2\r\nmamba install bioconda::trinity\r\nmamba install bioconda::stringtie\r\nmamba install bioconda::pasa\r\nmamba install bioconda::samtools\r\nmamba install bioconda::miniprot\r\nmamba install bioconda::evidencemodeler\r\nmamba install bioconda::minimap2\r\n<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c72a082 elementor-widget elementor-widget-heading\" data-id=\"c72a082\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">3. \u7ed3\u6784\u6ce8\u91ca<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2ca454b elementor-widget elementor-widget-heading\" data-id=\"2ca454b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">3.1 Denovo<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-76212d7 elementor-widget elementor-widget-code-highlight\" data-id=\"76212d7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>python annotation.py \\ \n--threads 20 \\\n--genome Final_genome_complete_sort.fasta \\\n--lineage Embryophyta \\\n--output Final_genome_complete_sort_denovo.gff\n\n# --threads\uff1aCPU \u6570\u91cf\n# --genome\uff1a\u57fa\u56e0\u7ec4\uff0c\u4e0d\u9700\u8981\u5c4f\u853d\n# --lineage\uff1aBUSCO\/Annotation \u53c2\u8003\u6570\u636e\u5e93\n# --output\uff1a\u8f93\u51fa GFF\n\n<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2e23222 elementor-widget elementor-widget-heading\" data-id=\"2e23222\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">3.2 RNA-seq<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c3cfefd elementor-widget elementor-widget-heading\" data-id=\"c3cfefd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">\u5168\u957f\u8f6c\u5f55\u7ec4\u6bd4\u5bf9\u5230\u57fa\u56e0\u7ec4<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-85de096 elementor-widget elementor-widget-code-highlight\" data-id=\"85de096\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>minimap2 -ax splice \\\n  -uf \\\n  -t 20 \\\n  ref.fa \\\n  ont.filtered.fq.gz \\\n| samtools sort \\\n  -@ 8 \\\n  -o ont.sorted.bam\n  \n# -ax \u6bd4\u5bf9\u7684\u6a21\u5f0f\uff0c\u8fd9\u91cc\u662f\u957freads\u6bd4\u5bf9\n# -uf \u8bbe\u7f6e GT-AG \u7684\u683c\u5f0f\uff0cf \u4e3a\u6807\u51c6\u7684\u8f6c\u5f55\u7ec4\u683c\u5f0f\n# -t 20 \u6bd4\u5bf9\u7684\u7ebf\u7a0b\u6570\n# -@ \u6392\u5e8f\u7684\u7ebf\u7a0b\u6570<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-22f57ce elementor-widget elementor-widget-heading\" data-id=\"22f57ce\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">\u7ec4\u88c5\u4e09\u4ee3\u8f6c\u5f55\u672c<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5463ea9 elementor-widget elementor-widget-code-highlight\" data-id=\"5463ea9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>stringtie  -p 40 \\\n-o  stringtie.gtf \\\n-L \\ \nDH2-YL1.bam DH2-OL1.bam\n\n# -p \u7ebf\u7a0b\u6570\n# -o \u8f6c\u5f55\u672cgff\u6587\u4ef6\n# -L \u957f\u8bfb\u957f\u6a21\u5f0f\n\n# \u63d0\u53d6\u8f6c\u5f55\u672c\ngtf_genome_to_cdna_fasta.pl \\\nstringtie.gtf \\\n..\/Final_genome_complete_sort.fasta > stringtie.fasta\n\n# -x \u53c2\u8003\u57fa\u56e0\u7ec4\n# -1 -2 \u53cc\u7aef\u6d4b\u5e8f\u6587\u4ef6\n# -o \u6bd4\u5bf9\u7ed3\u679c\n\n<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-514ee70 elementor-widget elementor-widget-heading\" data-id=\"514ee70\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">\u7ec4\u88c5\u4e8c\u4ee3\u8f6c\u5f55\u672c<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2c26699 elementor-widget elementor-widget-code-highlight\" data-id=\"2c26699\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>hisat2 --new-summary \/\n--summary-file rnaseq.hisat.summary\/\n--threads 20 \/\n-x ..\/Final_genome_complete_sort.fasta \/\n-1 Conbine_1.fq.gz \/\n-2 Conbine_2.fq.gz \/\n| samtools sort -@ 20 \/\n-o rnaseq.bam -\n\n# -x \u53c2\u8003\u57fa\u56e0\u7ec4\n# -1 -2 \u53cc\u7aef\u6d4b\u5e8f\u6587\u4ef6\n# -o \u6bd4\u5bf9\u7ed3\u679c\n\n# \u7ec4\u88c5\u8f6c\u5f55\u672c\nTrinity --max_memory 100G \/\n--genome_guided_bam .\/rnaseq.bam \/\n--genome_guided_max_intron 20000 \/\n--CPU 10 \/\n--output Trinity_result\n\n# --max_memory \u6700\u5927\u5185\u5b58\n# --genome_guided_bam \u8f93\u5165\u7684bam\u6587\u4ef6\n# --genome_guided_max_intron \u5185\u542b\u5b50\u6700\u5927\u7684\u957f\u5ea6\n# --CPU \u8fd0\u884c\u7684\u7ebf\u7a0b\u6570\n# --output \u8f93\u51fa\u6587\u4ef6\u5939<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bdac2df elementor-widget elementor-widget-heading\" data-id=\"bdac2df\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">\u5408\u5e76\u4e8c\u4e09\u4ee3\u8f6c\u5f55\u672c\uff0c\u5e76\u4e14\u4f7f\u7528PASA\u8fdb\u884c\u8f6c\u5f55\u672c\u7684\u7ec4\u88c5<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a0adb1e elementor-widget elementor-widget-code-highlight\" data-id=\"a0adb1e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>cat stringtie.fasta .\/Trinity_result\/Trinity-GG.fasta > Merge_transcript.fasta\n\nLaunch_PASA_pipeline.pl -c pasa.alignAssembly.Template.txt \/\n-C \/\n-R \/\n-g Final_genome_complete_sort.fasta \/\n-t Merge_transcript.fasta \/\n--ALIGNERS minimap2 \/\n--CPU 20\n\n# -g \u57fa\u56e0\u7ec4\u6587\u4ef6\n# -t \u8f6c\u5f55\u672c\uff0c\u8fd9\u91cc\u5e94\u8be5\u662f2+3\u7684\n# --ALIGNERS \u8f6c\u5f55\u672c\u548c\u57fa\u56e0\u7ec4\u7684\u6bd4\u5bf9\u8f6f\u4ef6\n# --CPU \u8fd0\u884c\u7684\u7ebf\u7a0b\u6570\n# -c \u914d\u7f6e\u6587\u4ef6\n\n<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-808f580 elementor-widget elementor-widget-heading\" data-id=\"808f580\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">pasa\u7684\u914d\u7f6e\u6587\u4ef6<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-17ece69 elementor-widget elementor-widget-code-highlight\" data-id=\"17ece69\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>## templated variables to be replaced exist as <__var_name__>\n\n# database settings\nDATABASE=\/home\/majunpeng\/sda2\/Xianyu\/Xigua\/Xigua\/Genome_asmmbly\/Anno\/Gene\/Final_genome_complete_sort.fasta.sqlit\n\n#######################################################\n# Parameters to specify to specific scripts in pipeline\n# create a key = \"script_name\" + \":\" + \"parameter\"\n# assign a value as done above.\n\n#script validate_alignments_in_db.dbi\nvalidate_alignments_in_db.dbi:--MIN_PERCENT_ALIGNED=80\nvalidate_alignments_in_db.dbi:--MIN_AVG_PER_ID=95\n\n#script subcluster_builder.dbi\nsubcluster_builder.dbi:-m=50<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cb59676 elementor-widget elementor-widget-heading\" data-id=\"cb59676\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">2.3 EVM\u8bc1\u636e\u6574\u5408<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7eea67a elementor-widget elementor-widget-text-editor\" data-id=\"7eea67a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u5230\u8fd9\u4e00\u6b65\uff0c\u5c31\u662f\u8981\u6574\u5408\u6765\u81eadenovo\uff0c\u540c\u6e90\u548cRNA-seq\u7684\u4e09\u65b9\u6ce8\u91ca\u7ed3\u679c\u3002\u5176\u4e2d\u540c\u6e90\uff0c\u4e5f\u5c31\u662fminiprot\u7684\u7ed3\u679c\u9700\u8981\u8fdb\u884c\u683c\u5f0f\u8f6c\u6362\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2a08770 elementor-widget elementor-widget-code-highlight\" data-id=\"2a08770\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>python3 miniprot_GFF_2_EVM_alignment_GFF3.py miniprot.gff > EVM_miniprot.gff<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0ddb249 elementor-widget elementor-widget-text-editor\" data-id=\"0ddb249\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u6b64\u5916\u8fd8\u8981\u6574\u7406\u4e00\u4e2a\u914d\u7f6e\u6587\u4ef6\uff0c\u8bbe\u5b9a\u6bcf\u79cd\u8bc1\u636e\u7684\u6743\u91cd<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-99cd898 elementor-widget elementor-widget-code-highlight\" data-id=\"99cd898\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>PROTEIN miniprot_protAln 3\nTRANSCRIPT assembler-Bra-pasa.sqlite 10\nABINITIO_PREDICTION ANNEVO 5 <\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-935fe2d elementor-widget elementor-widget-text-editor\" data-id=\"935fe2d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u8bc1\u636e\u6574\u5408<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9a5c930 elementor-widget elementor-widget-code-highlight\" data-id=\"9a5c930\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>EVidenceModeler --CPU 40 \\\n--sample EVM \\\n--genome Final_genome_complete_sort.fasta \\\n--weights EVM_Weight.txt \\\n--gene_predictions Final_genome_complete_sort_denovo.gff \\\n--protein_alignments EVM_miniprot.gff \\\n--transcript_alignments Final_genome_complete_sort.fasta.sqlit.pasa_assemblies.gff3 \\\n--segmentSize 100000 \\\n--overlapSize 10000 \\\n--repeats ..\/Final_genome_complete_sort.fasta.out.gff\n\n# --sample \u8f93\u51fa\u7ed3\u679c\u524d\u7f00\n# --genome \u57fa\u56e0\u7ec4\n# --weights \u6743\u91cd\u6587\u4ef6\n# --gene_predictions Dedovo\u9884\u6d4bgff\u6587\u4ef6\n# --protein_alignments \u540c\u6e90\u9884\u6d4bgff\u6587\u4ef6\n# --transcript_alignments RNA-seq\u9884\u6d4bgff\u6587\u4ef6\n# --repeats \u91cd\u590d\u5e8f\u5217\u6ce8\u91cagff\u6587\u4ef6\n# --segmentSize \u57fa\u56e0\u7ec4\u5212\u5206\u6210\u591a\u4e2a\u7247\u6bb5\uff0c\u6bcf\u4e2a\u7247\u6bb5\u7684\u5927\u5c0f\n# --overlapSize \u76f8\u90bb\u5206\u5757\u95f4\u4fdd\u7559\u7684\u91cd\u53e0\u533a\u57df\u5927\u5c0f<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>1. \u524d\u8a00 \u53bb\u5e74\u5e74\u521d\uff0c\u9996\u6b21\u5b8c\u6210\u4e86\u7b2c\u4e00\u4e2a\u7ec4\u88c5\u7684\u57fa\u56e0\u7ec4\u7684\u7ed3\u6784\u6ce8\u91ca\uff0c\u5e76\u4e14\u628a\u6d41\u7a0b\u8bb0\u5f55\u4e0b\u6765\u5199\u6210\u4e86\u535a\u5ba2\uff0c\u7f8e\u4e2d\u4e0d\u8db3\u662f\u57fa\u4e8e\u4e8c\u4ee3 &#8230; <a title=\"\u4f7f\u7528\u5168\u957f\u8f6c\u5f55\u7ec4\u8fdb\u884c\u57fa\u56e0\u7ec4\u7ed3\u6784\u6ce8\u91ca\" class=\"read-more\" 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