{"id":1848,"date":"2025-07-08T10:46:13","date_gmt":"2025-07-08T02:46:13","guid":{"rendered":"http:\/\/www.majunpeng.com\/?p=1848"},"modified":"2025-07-08T11:30:21","modified_gmt":"2025-07-08T03:30:21","slug":"purge_dups-%e5%9f%ba%e5%9b%a0%e7%bb%84%e4%b8%ad%e5%86%97%e4%bd%99%e7%9a%84%e5%ba%8f%e5%88%97","status":"publish","type":"post","link":"http:\/\/www.majunpeng.com\/index.php\/2025\/07\/08\/purge_dups-%e5%9f%ba%e5%9b%a0%e7%bb%84%e4%b8%ad%e5%86%97%e4%bd%99%e7%9a%84%e5%ba%8f%e5%88%97\/","title":{"rendered":"Purge_dups \u57fa\u56e0\u7ec4\u4e2d\u5197\u4f59\u7684\u5e8f\u5217"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"1848\" class=\"elementor elementor-1848\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c8ea7d1 e-flex e-con-boxed e-con e-parent\" data-id=\"c8ea7d1\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-169071e elementor-widget elementor-widget-heading\" data-id=\"169071e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">1. \u524d\u8a00<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c505d6a elementor-widget elementor-widget-text-editor\" data-id=\"c505d6a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u63a5\u4e86\u4e00\u4e2a\u57fa\u56e0\u7ec4\u7ec4\u88c5\u7684\u9879\u76ee\uff0c\u4f46\u662f\u6d4b\u5e8f\u7684\u65f6\u5019\u662f\u6df7\u6837\u6d4b\u5f97\uff0c\u5bfc\u81f4\u7ec4\u88c5\u7684\u57fa\u56e0\u7ec4\u628a\u4e24\u4e2a\u6837\u54c1\u7684\u57fa\u56e0\u7ec4\u90fd\u7ec4\u88c5\u51fa\u6765\u4e86\uff0c\u53ea\u80fd\u7528Purge_dups\u53bb\u5197\u4f59\u4e86\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4cb67f1 elementor-widget elementor-widget-heading\" data-id=\"4cb67f1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">2. \u5b89\u88c5<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e4dd3d6 elementor-widget elementor-widget-code-highlight\" data-id=\"e4dd3d6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>git clone https:\/\/github.com\/dfguan\/purge_dups.git\ncd purge_dups\/src\nmake<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aac76fa elementor-widget elementor-widget-heading\" data-id=\"aac76fa\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">3. \u8fd0\u884c<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-745b6ef elementor-widget elementor-widget-code-highlight\" data-id=\"745b6ef\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp> # \u6574\u4e2a\u6d41\u7a0b\u53ef\u4ee5\u5206\u4e3a\u4ee5\u4e0b\u51e0\u4e2a\u4e3b\u8981\u6b65\u9aa4\uff1a\n   1. PacBio HiFi \u6d4b\u5e8f\u6570\u636e\u56de\u6bd4\u5bf9\uff1a\u5c06\u957f\u8bfb\u957f\uff08PacBio\uff09\u6d4b\u5e8f\u6570\u636e\u6bd4\u5bf9\u56de\u521d\u6b65\u7ec4\u88c5\u7684\u57fa\u56e0\u7ec4\u4e0a\uff0c\u7528\u4e8e\u8ba1\u7b97\u6d4b\u5e8f\u6df1\u5ea6\u3002\n   2. \u8ba1\u7b97\u6d4b\u5e8f\u6df1\u5ea6\u548c\u8986\u76d6\u5ea6\uff1a\u4f7f\u7528 pbcstat \u5de5\u5177\u5206\u6790\u6bd4\u5bf9\u7ed3\u679c\uff0c\u751f\u6210\u8986\u76d6\u5ea6\u7edf\u8ba1\u4fe1\u606f\u3002\n   3. \u786e\u5b9a\u6df1\u5ea6\u9608\u503c\uff1a\u4f7f\u7528 calcuts \u5de5\u5177\u6839\u636e\u6df1\u5ea6\u5206\u5e03\uff0c\u81ea\u52a8\u8ba1\u7b97\u7528\u4e8e\u533a\u5206\u6b63\u5e38\u5e8f\u5217\u3001\u91cd\u590d\u5e8f\u5217\u548c\u5783\u573e\u5e8f\u5217\u7684\u6df1\u5ea6\u9608\u503c\u3002\n   4. \u57fa\u56e0\u7ec4\u81ea\u8eab\u6bd4\u5bf9\uff1a\u5c06\u7ec4\u88c5\u597d\u7684\u57fa\u56e0\u7ec4\u5e8f\u5217\u81ea\u5df1\u548c\u81ea\u5df1\u6bd4\u5bf9\uff0c\u4ee5\u8bc6\u522b\u7ed3\u6784\u4e0a\u76f8\u4f3c\u7684\u533a\u57df\uff08\u5373\u6f5c\u5728\u7684\u91cd\u590d\u533a\uff09\u3002\n   5. \u8bc6\u522b\u5e76\u7b5b\u9009\u91cd\u590d\u5e8f\u5217\uff1a\u7ed3\u5408\u6df1\u5ea6\u9608\u503c\u548c\u81ea\u8eab\u6bd4\u5bf9\u7ed3\u679c\uff0c\u4f7f\u7528 purge_dups \u4e3b\u7a0b\u5e8f\u8bc6\u522b\u51fa\u54ea\u4e9b\u662f\u5197\u4f59\u5e8f\u5217\u3002\n   6. \u63d0\u53d6\u6700\u7ec8\u5e8f\u5217\uff1a\u6839\u636e\u4e0a\u4e00\u6b65\u7684\u8bc6\u522b\u7ed3\u679c\uff0c\u4ece\u539f\u59cb\u7ec4\u88c5\u6587\u4ef6\u4e2d\u79fb\u9664\u5197\u4f59\u5e8f\u5217\uff0c\u4fdd\u7559\u4e3b\u5e8f\u5217\uff0c\u751f\u6210\u6700\u7ec8\u7684\u7eaf\u51c0\u7248\u57fa\u56e0\u7ec4\u3002\n\n1. PacBio HiFi \u6570\u636e\u56de\u6bd4\u5bf9\n\n\n\n   1 minimap2 -t 60 -x map-pb final_asm.bp.p_ctg.fa out.fasta.gz | gzip -c - > pb_aln.paf.gz\n\n\n\n   * \u547d\u4ee4: minimap2\n   * \u76ee\u7684: \u5c06 PacBio HiFi \u6d4b\u5e8f\u6570\u636e\uff08out.fasta.gz\uff09\u6bd4\u5bf9\u5230\u521d\u6b65\u7ec4\u88c5\u597d\u7684\u57fa\u56e0\u7ec4\uff08final_asm.bp.p_ctg.fa\uff09\u4e0a\u3002\u8fd9\u662f\u4e3a\u4e86\u540e\u7eed\u8ba1\u7b97\u6bcf\u4e2acontig\uff08\u62fc\u63a5\u5e8f\u5217\uff09\u7684\u6d4b\u5e8f\u6df1\u5ea6\u3002\n   * \u53c2\u6570:\n       * -t 60: \u4f7f\u7528 60 \u4e2a\u7ebf\u7a0b\u8fdb\u884c\u8ba1\u7b97\uff0c\u4ee5\u52a0\u5feb\u901f\u5ea6\u3002\n       * -x map-pb: \u4f7f\u7528 minimap2 \u9884\u8bbe\u7684\u53c2\u6570\u96c6\uff0c\u4e13\u95e8\u7528\u4e8e\u5c06 PacBio \u6d4b\u5e8f\u6570\u636e\u6bd4\u5bf9\u5230\u53c2\u8003\u57fa\u56e0\u7ec4\u3002\n   * \u8f93\u5165\u6587\u4ef6:\n       * final_asm.bp.p_ctg.fa: \u53c2\u8003\u5e8f\u5217\uff0c\u5373\u521d\u6b65\u7ec4\u88c5\u597d\u7684\u57fa\u56e0\u7ec4\u6587\u4ef6\u3002\n       * out.fasta.gz: \u67e5\u8be2\u5e8f\u5217\uff0c\u5373 PacBio HiFi \u6d4b\u5e8f\u7684 reads \u6587\u4ef6\uff08FASTQ \u6216 FASTA \u683c\u5f0f\uff0c\u6b64\u5904\u4e3a .gz \u538b\u7f29\u683c\u5f0f\uff09\u3002\n   * \u8f93\u51fa\u6587\u4ef6:\n       * pb_aln.paf.gz: \u6bd4\u5bf9\u7ed3\u679c\u6587\u4ef6\uff0c\u683c\u5f0f\u4e3a PAF (Pairwise mApping Format)\u3002\u901a\u8fc7\u7ba1\u9053\uff08|\uff09\u548c gzip \u547d\u4ee4\u8fdb\u884c\u538b\u7f29\uff0c\u8282\u7701\u5b58\u50a8\u7a7a\u95f4\u3002\n\n  2. \u8ba1\u7b97\u8986\u76d6\u5ea6\u7edf\u8ba1\n\n\n\n   1 ~\/Software\/purge_dups\/bin\/pbcstat pb_aln.paf.gz\n\n\n\n   * \u547d\u4ee4: pbcstat\n   * \u76ee\u7684: \u5206\u6790\u4e0a\u4e00\u6b65\u751f\u6210\u7684 PAF \u6bd4\u5bf9\u6587\u4ef6\uff0c\u8ba1\u7b97\u57fa\u56e0\u7ec4\u4e2d\u6bcf\u4e2a\u4f4d\u7f6e\u7684\u6d4b\u5e8f\u6df1\u5ea6\u548c\u8986\u76d6\u5ea6\u7edf\u8ba1\u4fe1\u606f\u3002\n   * \u8f93\u5165\u6587\u4ef6:\n       * pb_aln.paf.gz: minimap2 \u751f\u6210\u7684\u6bd4\u5bf9\u6587\u4ef6\u3002\n   * \u8f93\u51fa\u6587\u4ef6:\n       * PB.stat: \u9ed8\u8ba4\u8f93\u51fa\u6587\u4ef6\uff0c\u5305\u542b\u4e86\u6bcf\u4e2a contig \u7684\u8986\u76d6\u5ea6\u7edf\u8ba1\u4fe1\u606f\u3002\n       * PB.base.cov: \u9ed8\u8ba4\u8f93\u51fa\u6587\u4ef6\uff0c\u5305\u542b\u4e86\u57fa\u56e0\u7ec4\u6bcf\u4e2a\u78b1\u57fa\u7684\u8986\u76d6\u6df1\u5ea6\u3002\n\n  3. \u8ba1\u7b97\u6df1\u5ea6\u9608\u503c\n\n\n\n   1 ~\/Software\/purge_dups\/bin\/calcuts PB.stat > cutoffs 2> calcults.log\n\n\n\n   * \u547d\u4ee4: calcuts\n   * \u76ee\u7684: \u57fa\u4e8e pbcstat\n     \u751f\u6210\u7684\u7edf\u8ba1\u6587\u4ef6\uff0c\u81ea\u52a8\u8ba1\u7b97\u548c\u8bbe\u5b9a\u6d4b\u5e8f\u6df1\u5ea6\u7684\u5206\u754c\u70b9\uff08cutoffs\uff09\u3002\u5b83\u4f1a\u6839\u636e\u6df1\u5ea6\u5206\u5e03\u56fe\uff0c\u4f30\u7b97\u51fa\u6b63\u5e38\u5355\u500d\u578b\u5e8f\u5217\u7684\u5e73\u5747\u6df1\u5ea6\uff0c\u5e76\u4ee5\u6b64\u4e3a\u4f9d\u636e\u8bbe\u5b9a\u4e00\u4e2a\u4e0a\u9650\u548c\u4e0b\u9650\uff0c\u7528\u4e8e\u533a\u5206\u6b63\u5e38\u5e8f\u5217\u548c\u91cd\u590d\u5e8f\u5217\uff08\u901a\u5e38\u6df1\u5ea6\u66f4\u9ad8\uff09\u3002\n   * \u8f93\u5165\u6587\u4ef6:\n       * PB.stat: pbcstat \u751f\u6210\u7684\u7edf\u8ba1\u6587\u4ef6\u3002\n   * \u8f93\u51fa\u6587\u4ef6:\n       * cutoffs: \u5305\u542b\u4e86\u8ba1\u7b97\u51fa\u7684\u6df1\u5ea6\u9608\u503c\u3002\u8fd9\u4e2a\u6587\u4ef6\u4f1a\u88ab\u540e\u7eed\u7684 purge_dups \u547d\u4ee4\u4f7f\u7528\u3002\n       * calcults.log: \u8bb0\u5f55 calcuts \u8fd0\u884c\u8fc7\u7a0b\u4e2d\u7684\u65e5\u5fd7\u4fe1\u606f\uff08\u901a\u8fc7 2> \u91cd\u5b9a\u5411\u6807\u51c6\u9519\u8bef\u6d41\uff09\u3002\n\n  4. \u57fa\u56e0\u7ec4\u81ea\u8eab\u6bd4\u5bf9\n\n\n\n   1 ~\/Software\/purge_dups\/bin\/split_fa final_asm.bp.p_ctg.fa > asm.split\n   2 minimap2 -t 60 -xasm5 -DP asm.split asm.split | pigz -c > asm.split.self.paf.gz\n\n\n\n   * \u7b2c\u4e00\u90e8\u5206: split_fa\n       * \u76ee\u7684: \u5c06\u539f\u59cb\u7684\u57fa\u56e0\u7ec4 FASTA \u6587\u4ef6\u8fdb\u884c\u5207\u5206\u6216\u683c\u5f0f\u5316\uff0c\u4f7f\u5176\u9002\u5408\u540e\u7eed\u7684\u81ea\u8eab\u6bd4\u5bf9\u3002\n       * \u8f93\u5165: final_asm.bp.p_ctg.fa\n       * \u8f93\u51fa: asm.split (\u683c\u5f0f\u5316\u540e\u7684 FASTA \u6587\u4ef6)\n   * \u7b2c\u4e8c\u90e8\u5206: minimap2\n       * \u76ee\u7684: \u5c06\u57fa\u56e0\u7ec4\u4e0e\u81ea\u8eab\u8fdb\u884c\u6bd4\u5bf9\uff0c\u4ee5\u627e\u51fa\u6240\u6709\u76f8\u4f3c\u5ea6\u9ad8\u7684\u533a\u57df\u3002\u8fd9\u4e9b\u533a\u57df\u662f\u6f5c\u5728\u7684\u91cd\u590d\u5e8f\u5217\u6216\u5355\u500d\u578b\u5e8f\u5217\u3002\n       * \u53c2\u6570:\n           * -t 60: \u4f7f\u7528 60 \u4e2a\u7ebf\u7a0b\u3002\n           * -x asm5: \u4f7f\u7528\u9884\u8bbe\u53c2\u6570\u96c6\uff0c\u9002\u7528\u4e8e\u9ad8\u8d28\u91cf\u57fa\u56e0\u7ec4\uff08\u9519\u8bef\u7387 <5%\uff09\u4e4b\u95f4\u7684\u6bd4\u5bf9\u3002\n           * -DP: -D \u6807\u8bb0\u4e3b\u94fe\uff08primary chain\uff09\uff0c-P \u4fdd\u7559\u6b21\u8981\u94fe\uff08secondary chains\uff09\uff0c\u6709\u52a9\u4e8e\u8bc6\u522b\u6240\u6709\u53ef\u80fd\u7684\u91cd\u53e0\u3002\n       * \u8f93\u5165\u6587\u4ef6:\n           * asm.split: \u4e24\u6b21\u4f5c\u4e3a\u8f93\u5165\uff0c\u8868\u793a\u5c06\u8fd9\u4e2a\u6587\u4ef6\u4e0e\u5b83\u81ea\u8eab\u8fdb\u884c\u6bd4\u5bf9\u3002\n       * \u8f93\u51fa\u6587\u4ef6:\n           * asm.split.self.paf.gz: \u81ea\u8eab\u6bd4\u5bf9\u7684\u7ed3\u679c\uff0c\u4ee5 PAF \u683c\u5f0f\u5b58\u50a8\uff0c\u5e76\u7528 pigz\uff08\u4e00\u4e2a\u5e76\u884c\u7684 gzip \u5de5\u5177\uff09\u8fdb\u884c\u538b\u7f29\u3002\n\n\n  5. \u8bc6\u522b\u5197\u4f59\u5e8f\u5217\n\n\n\n   1 ~\/Software\/purge_dups\/bin\/purge_dups -2 -T cutoffs -c PB.base.cov asm.split.self.paf.gz > dups.bed 2> purge_dups.log\n\n\n\n   * \u547d\u4ee4: purge_dups\n   * \u76ee\u7684: \u8fd9\u662f\u6838\u5fc3\u6b65\u9aa4\u3002\u5b83\u6574\u5408\u4e86\u6d4b\u5e8f\u6df1\u5ea6\u4fe1\u606f\u548c\u81ea\u8eab\u6bd4\u5bf9\u7ed3\u679c\uff0c\u6765\u6700\u7ec8\u5224\u65ad\u54ea\u4e9b\u5e8f\u5217\u662f\u5197\u4f59\u7684\u3002\n   * \u53c2\u6570:\n       * -2: \u8868\u793a\u4f7f\u7528 purge_dups \u7684\u7b2c\u4e8c\u7248\u7b97\u6cd5\uff08\u901a\u5e38\u662f\u9ed8\u8ba4\u6216\u63a8\u8350\u7684\uff09\u3002\n       * -T cutoffs: \u6307\u5b9a\u5305\u542b\u6df1\u5ea6\u9608\u503c\u7684\u6587\u4ef6\uff0c\u5373 calcuts \u547d\u4ee4\u751f\u6210\u7684 cutoffs \u6587\u4ef6\u3002\n       * -c PB.base.cov: \u6307\u5b9a\u6bcf\u4e2a\u78b1\u57fa\u7684\u8986\u76d6\u5ea6\u6587\u4ef6\uff0c\u5373 pbcstat \u751f\u6210\u7684 PB.base.cov \u6587\u4ef6\u3002\n   * \u8f93\u5165\u6587\u4ef6:\n       * asm.split.self.paf.gz: \u57fa\u56e0\u7ec4\u81ea\u8eab\u6bd4\u5bf9\u7684\u7ed3\u679c\u3002\n       * cutoffs: \u6df1\u5ea6\u9608\u503c\u6587\u4ef6\u3002\n       * PB.base.cov: \u78b1\u57fa\u8986\u76d6\u5ea6\u6587\u4ef6\u3002\n   * \u8f93\u51fa\u6587\u4ef6:\n       * dups.bed: \u4e00\u4e2a BED \u683c\u5f0f\u7684\u6587\u4ef6\uff0c\u5217\u51fa\u4e86\u6240\u6709\u88ab\u8bc6\u522b\u4e3a\u5197\u4f59\uff08duplication\uff09\u7684\u5e8f\u5217\u7684\u5750\u6807\u3002\n       * purge_dups.log: \u8bb0\u5f55 purge_dups \u8fd0\u884c\u8fc7\u7a0b\u7684\u65e5\u5fd7\u3002\n\n  6. \u63d0\u53d6\u6700\u7ec8\u7684\u975e\u5197\u4f59\u5e8f\u5217\n\n\n   * \u547d\u4ee4: purge_dups\n   * \u76ee\u7684: \u8fd9\u662f\u6838\u5fc3\u6b65\u9aa4\u3002\u5b83\u6574\u5408\u4e86\u6d4b\u5e8f\u6df1\u5ea6\u4fe1\u606f\u548c\u81ea\u8eab\u6bd4\u5bf9\u7ed3\u679c\uff0c\u6765\u6700\u7ec8\u5224\u65ad\u54ea\u4e9b\u5e8f\u5217\u662f\u5197\u4f59\u7684\u3002\n   * \u53c2\u6570:\n\n   1 ~\/Software\/purge_dups\/bin\/get_seqs dups.bed final_asm.bp.p_ctg.fa\n\n\n\n   * \u547d\u4ee4: get_seqs\n   * \u76ee\u7684: \u6839\u636e purge_dups \u751f\u6210\u7684 dups.bed \u6587\u4ef6\uff0c\u4ece\u539f\u59cb\u7684\u7ec4\u88c5\u57fa\u56e0\u7ec4\u4e2d\u79fb\u9664\u8fd9\u4e9b\u88ab\u6807\u8bb0\u4e3a\u5197\u4f59\u7684\u5e8f\u5217\uff0c\u5e76\u8f93\u51fa\u4e00\u4e2a\u7eaf\u51c0\u7248\u7684\u57fa\u56e0\u7ec4\u3002\n   * \u8f93\u5165\u6587\u4ef6:\n       * dups.bed: \u5305\u542b\u5197\u4f59\u5e8f\u5217\u5750\u6807\u7684 BED \u6587\u4ef6\u3002\n       * final_asm.bp.p_ctg.fa: \u539f\u59cb\u7684\u3001\u672a\u7ecf\u8fc7\u6ee4\u7684\u7ec4\u88c5\u57fa\u56e0\u7ec4\u3002\n   * \u8f93\u51fa\u6587\u4ef6:\n       * \u9ed8\u8ba4\u60c5\u51b5\u4e0b\uff0c\u5b83\u4f1a\u751f\u6210\u4e24\u4e2a\u6587\u4ef6\uff1a\n           * purged.fa: \u6700\u7ec8\u7684\u3001\u53bb\u9664\u4e86\u5197\u4f59\u5e8f\u5217\u7684\u57fa\u56e0\u7ec4\u6587\u4ef6\u3002\u8fd9\u662f\u6574\u4e2a\u6d41\u7a0b\u6700\u91cd\u8981\u7684\u4ea7\u51fa\u6587\u4ef6\u3002\n           * hap.fa: \u88ab\u8bc6\u522b\u4e3a\u5197\u4f59\u6216\u5355\u500d\u578b\uff08haplotigs\uff09\u7684\u5e8f\u5217\u6587\u4ef6\u3002<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>1. \u524d\u8a00 \u63a5\u4e86\u4e00\u4e2a\u57fa\u56e0\u7ec4\u7ec4\u88c5\u7684\u9879\u76ee\uff0c\u4f46\u662f\u6d4b\u5e8f\u7684\u65f6\u5019\u662f\u6df7\u6837\u6d4b\u5f97\uff0c\u5bfc\u81f4\u7ec4\u88c5\u7684\u57fa\u56e0\u7ec4\u628a\u4e24\u4e2a\u6837\u54c1\u7684\u57fa\u56e0\u7ec4\u90fd\u7ec4\u88c5\u51fa\u6765\u4e86 &#8230; <a title=\"Purge_dups \u57fa\u56e0\u7ec4\u4e2d\u5197\u4f59\u7684\u5e8f\u5217\" class=\"read-more\" href=\"http:\/\/www.majunpeng.com\/index.php\/2025\/07\/08\/purge_dups-%e5%9f%ba%e5%9b%a0%e7%bb%84%e4%b8%ad%e5%86%97%e4%bd%99%e7%9a%84%e5%ba%8f%e5%88%97\/\" aria-label=\"\u9605\u8bfb Purge_dups \u57fa\u56e0\u7ec4\u4e2d\u5197\u4f59\u7684\u5e8f\u5217\">\u9605\u8bfb\u66f4\u591a<\/a><\/p>\n","protected":false},"author":1,"featured_media":1849,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_crdt_document":"","footnotes":""},"categories":[6],"tags":[],"class_list":["post-1848","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-6"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Purge_dups \u57fa\u56e0\u7ec4\u4e2d\u5197\u4f59\u7684\u5e8f\u5217 - 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