{"id":1726,"date":"2025-06-23T11:38:42","date_gmt":"2025-06-23T03:38:42","guid":{"rendered":"http:\/\/www.majunpeng.com\/?p=1726"},"modified":"2025-06-23T11:50:05","modified_gmt":"2025-06-23T03:50:05","slug":"ragtag%ef%bc%9a%e6%8a%8a%e4%bd%a0%e7%9a%84%e5%9f%ba%e5%9b%a0%e7%bb%84%e6%8f%90%e5%8d%87%e8%87%b3%e6%9f%93%e8%89%b2%e4%bd%93%e6%b0%b4%e5%b9%b3","status":"publish","type":"post","link":"http:\/\/www.majunpeng.com\/index.php\/2025\/06\/23\/ragtag%ef%bc%9a%e6%8a%8a%e4%bd%a0%e7%9a%84%e5%9f%ba%e5%9b%a0%e7%bb%84%e6%8f%90%e5%8d%87%e8%87%b3%e6%9f%93%e8%89%b2%e4%bd%93%e6%b0%b4%e5%b9%b3\/","title":{"rendered":"Ragtag\uff1a\u628a\u4f60\u7684\u57fa\u56e0\u7ec4\u63d0\u5347\u81f3\u67d3\u8272\u4f53\u6c34\u5e73"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"1726\" class=\"elementor elementor-1726\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6fd191e e-flex e-con-boxed e-con e-parent\" data-id=\"6fd191e\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-9ec4ddd elementor-widget elementor-widget-heading\" data-id=\"9ec4ddd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">1. \u524d\u8a00<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-da96e66 elementor-widget elementor-widget-text-editor\" data-id=\"da96e66\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u76ee\u524d\u6700\u65b0\u53d1\u8868\u7684\u57fa\u56e0\u7ec4\u5927\u591a\u90fd\u8fbe\u5230\u4e86\u67d3\u8272\u4f53\u6c34\u5e73\uff0c\u4f46\u662f\u4e00\u4e9b\u8001\u7684\u57fa\u56e0\u7ec4\u8fd8\u662fContig\u6216\u8005Scaffiold\u6c34\u5e73\uff0c\u5982\u679c\u60f3\u628a\u8fd9\u4e9b\u57fa\u56e0\u7ec4\u5347\u7ea7\u5230\u67d3\u8272\u4f53\u6c34\u5e73\u662f\u4e0d\u662f\u8981\u501f\u52a9HIC\u6570\u636e\u5462\uff1f\u7b54\u6848\u662fNO\uff0cRagtag\u53ef\u4ee5\u505a\u5230\uff0c\u901a\u8fc7\u628aContig\u6216\u8005Scaffold\u6bd4\u5bf9\u7684\u67d3\u8272\u4f53\u6c34\u5e73\u7684\u53c2\u8003\u57fa\u56e0\u7ec4\u4e0a\uff0c\u5347\u7ea7\u81f3\u67d3\u8272\u4f53\u6c34\u5e73\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-44d7c01 elementor-widget elementor-widget-heading\" data-id=\"44d7c01\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">2. \u4e0b\u8f7d\u8f6f\u4ef6<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7c7b620 elementor-widget elementor-widget-code-highlight\" data-id=\"7c7b620\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-javascript line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-javascript\">\n\t\t\t\t\t<xmp>mamba create -n Ragtag\nmamba activate Ragtag\nmamba install bioconda::ragtag <\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a204860 elementor-widget elementor-widget-heading\" data-id=\"a204860\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">3. \u8fd0\u884c<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3fe26d6 elementor-widget elementor-widget-text-editor\" data-id=\"3fe26d6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u57fa\u4e8e\u53c2\u8003\u57fa\u56e0\u7ec4\u77eb\u6b63\u7ec4\u88c5\u8fc7\u7a0b\u4ea7\u751f\u7684\u9519\u8bef<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-62a5578 elementor-widget elementor-widget-code-highlight\" data-id=\"62a5578\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-javascript line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-javascript\">\n\t\t\t\t\t<xmp>ragtag.py correct ref.fasta query.fasta -t 8<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-68af559 elementor-widget elementor-widget-text-editor\" data-id=\"68af559\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u6bd4\u5bf9\uff0c\u751f\u6210\u67d3\u8272\u4f53\u6c34\u5e73\u7684\u57fa\u56e0\u7ec4<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e6cefab elementor-widget elementor-widget-code-highlight\" data-id=\"e6cefab\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-javascript line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-javascript\">\n\t\t\t\t\t<xmp>ragtag.py scaffold ref.fa ragtag_output\/query.corrected.fasta<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-65ba75d elementor-widget elementor-widget-text-editor\" data-id=\"65ba75d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Gap\u586b\u8865<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-70a419b elementor-widget elementor-widget-code-highlight\" data-id=\"70a419b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-javascript line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-javascript\">\n\t\t\t\t\t<xmp>ragtag.py patch <target.fa> <query.fa><\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>1. \u524d\u8a00 \u76ee\u524d\u6700\u65b0\u53d1\u8868\u7684\u57fa\u56e0\u7ec4\u5927\u591a\u90fd\u8fbe\u5230\u4e86\u67d3\u8272\u4f53\u6c34\u5e73\uff0c\u4f46\u662f\u4e00\u4e9b\u8001\u7684\u57fa\u56e0\u7ec4\u8fd8\u662fContig\u6216\u8005Scaffiol &#8230; <a title=\"Ragtag\uff1a\u628a\u4f60\u7684\u57fa\u56e0\u7ec4\u63d0\u5347\u81f3\u67d3\u8272\u4f53\u6c34\u5e73\" class=\"read-more\" href=\"http:\/\/www.majunpeng.com\/index.php\/2025\/06\/23\/ragtag%ef%bc%9a%e6%8a%8a%e4%bd%a0%e7%9a%84%e5%9f%ba%e5%9b%a0%e7%bb%84%e6%8f%90%e5%8d%87%e8%87%b3%e6%9f%93%e8%89%b2%e4%bd%93%e6%b0%b4%e5%b9%b3\/\" aria-label=\"\u9605\u8bfb Ragtag\uff1a\u628a\u4f60\u7684\u57fa\u56e0\u7ec4\u63d0\u5347\u81f3\u67d3\u8272\u4f53\u6c34\u5e73\">\u9605\u8bfb\u66f4\u591a<\/a><\/p>\n","protected":false},"author":1,"featured_media":1727,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_crdt_document":"","footnotes":""},"categories":[6],"tags":[],"class_list":["post-1726","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-6"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ 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