{"id":1673,"date":"2025-06-13T00:25:09","date_gmt":"2025-06-12T16:25:09","guid":{"rendered":"http:\/\/www.majunpeng.com\/?p=1673"},"modified":"2025-06-20T01:20:25","modified_gmt":"2025-06-19T17:20:25","slug":"%e5%9f%ba%e5%9b%a0%e7%bb%84%e5%8d%95%e5%80%8d%e5%9e%8b%e5%88%86%e6%9e%90","status":"publish","type":"post","link":"http:\/\/www.majunpeng.com\/index.php\/2025\/06\/13\/%e5%9f%ba%e5%9b%a0%e7%bb%84%e5%8d%95%e5%80%8d%e5%9e%8b%e5%88%86%e6%9e%90\/","title":{"rendered":"\u57fa\u56e0\u7ec4\u5355\u500d\u578b\u5206\u6790"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"1673\" class=\"elementor elementor-1673\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ca41c2b e-flex e-con-boxed e-con e-parent\" data-id=\"ca41c2b\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-579781a elementor-widget elementor-widget-heading\" data-id=\"579781a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">1. \u524d\u8a00<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-35c767a elementor-widget elementor-widget-text-editor\" data-id=\"35c767a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u968f\u7740\u57fa\u56e0\u7ec4\u7ec4\u88c5\u7684\u6280\u672f\u4e0d\u65ad\u5b8c\u5584\uff0cT2T\u548c\u5355\u500d\u578b\u57fa\u56e0\u7ec4\u5f88\u5bb9\u6613\u7ec4\u88c5\u51fa\u6765\uff0c\u5355\u500d\u578b\u57fa\u56e0\u7ec4\u6bd4\u8f83\u5206\u6790\u4e5f\u662f\u8fd1\u671f\u6bd4\u8f83\u706b\u7684\uff0c\u56e0\u6b64\u6597\u80c6\u6765\u63a5\u7740\u8fd9\u4e2a\u673a\u4f1a\u6765\u5206\u6790\u4ee5\u4e0b\uff0c\u5927\u6982\u5c31\u662f\u4ee5\u4e0b\u4e24\u4e2a\u5206\u6790\uff081\uff09\u5355\u500d\u578b\u57fa\u56e0\u7ec4\u7684\u7ed3\u6784\u5dee\u5f02\u5206\u6790\uff082\uff09\u5355\u500d\u578b\u7b49\u4f4d\u57fa\u56e0\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u548c\u4e0b\u6e38\u7684\u5dee\u5f02\u57fa\u56e0\u5bcc\u96c6\u5206\u6790\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2e9b0e1 elementor-widget elementor-widget-heading\" data-id=\"2e9b0e1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">2. \u8f6f\u4ef6\u4e0b\u8f7d\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8500c9a elementor-widget elementor-widget-code-highlight\" data-id=\"8500c9a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>mamba create -n Hap_compritive\\\n\nmamba activate Hap_compritive\n\nmamba install bioconda::minimap2\n\nmamba install bioconda::syri\n\nmamba install bioconda::plotsr \n\ngit clone https:\/\/github.com\/zhangrengang\/orthoindex.git\ncd orthoindex\nmamba env create -f OrthoIndex.yaml\nmamba activate OrthoIndex\npython3 setup.py install\n\n<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-21e0386 elementor-widget elementor-widget-heading\" data-id=\"21e0386\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">3. \u5355\u500d\u578b\u7ed3\u6784\u53d8\u5f02\u5206\u6790\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ae422b2 elementor-widget elementor-widget-code-highlight\" data-id=\"ae422b2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp>minimap2 -ax asm5 --eqx  zhslhap1genome.fa zhslhap2genome.fa -t 40 > Minimap.sam\nsyri -c Minimap.sam -r zhslhap1genome.fa -q zhslhap2genome.fa -k -F S\nplotsr --sr syri.out --genomes genome.txt -o output_plot.png\n\n<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e4050b7 elementor-widget elementor-widget-heading\" data-id=\"e4050b7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">4. \u5355\u500d\u578b\u57fa\u56e0\u7ec4\u7b49\u4f4d\u57fa\u56e0\u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8b02beb elementor-widget elementor-widget-text-editor\" data-id=\"8b02beb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u6682\u65f6\u60f3\u5230\u7684\u7b56\u7565\u5c31\u662fjcvi\u9274\u5b9a\u5b9a\u4f4d\u57fa\u56e0\uff0c\u7136\u540eRSEM\u5b9a\u91cf\uff0c\u6700\u540e\u8fdb\u884c\u5dee\u5f02\u5206\u6790\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1eaac08 elementor-widget elementor-widget-code-highlight\" data-id=\"1eaac08\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"code-highlight.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"prismjs-okaidia copy-to-clipboard \">\n\t\t\t<pre data-line=\"\" class=\"highlight-height language-bash line-numbers\">\n\t\t\t\t<code readonly=\"true\" class=\"language-bash\">\n\t\t\t\t\t<xmp># \u63d0\u53d6\u8f6c\u5f55\u672c\u5e76\u6784\u5efa\u7d22\u5f15\nrsem-prepare-reference -p 20 -gff3 zhslhap2.gff3 --bowtie2 zhslhap1genome.fa zhslhap1genome\nrsem-prepare-reference -p 20 -gff3 zhslhap2.gff3 --bowtie2 zhslhap2genome.fa zhslhap2genome\n# \u6bd4\u5bf9\u5e76\u5b9a\u91cf\nrsem-calculate-expression --bowtie2 --paired-end -p 12 zhsl_1.fq zhsl_2.fq zhslhap1genome zhslhap1genome_rsem\nrsem-calculate-expression --bowtie2 --paired-end -p 12 zhsl_1.fq zhsl_2.fq zhslhap2genome zhslhap2genome_rsem\n\n<\/xmp>\n\t\t\t\t<\/code>\n\t\t\t<\/pre>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-353a97c elementor-widget elementor-widget-text-editor\" data-id=\"353a97c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u8f93\u51fa\u7ed3\u679c\u6bd4\u8f83\u91cd\u8981\u7684\u5c31\u662f*genes.result\u548c*isoforms.results\uff0c\u5206\u522b\u662f\u57fa\u56e0\u548c\u8f6c\u5f55\u672c\u7684\u5b9a\u91cf\u7ed3\u679c<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>1. \u524d\u8a00 \u968f\u7740\u57fa\u56e0\u7ec4\u7ec4\u88c5\u7684\u6280\u672f\u4e0d\u65ad\u5b8c\u5584\uff0cT2T\u548c\u5355\u500d\u578b\u57fa\u56e0\u7ec4\u5f88\u5bb9\u6613\u7ec4\u88c5\u51fa\u6765\uff0c\u5355\u500d\u578b\u57fa\u56e0\u7ec4\u6bd4\u8f83\u5206\u6790\u4e5f\u662f\u8fd1\u671f\u6bd4\u8f83 &#8230; <a title=\"\u57fa\u56e0\u7ec4\u5355\u500d\u578b\u5206\u6790\" class=\"read-more\" href=\"http:\/\/www.majunpeng.com\/index.php\/2025\/06\/13\/%e5%9f%ba%e5%9b%a0%e7%bb%84%e5%8d%95%e5%80%8d%e5%9e%8b%e5%88%86%e6%9e%90\/\" aria-label=\"\u9605\u8bfb \u57fa\u56e0\u7ec4\u5355\u500d\u578b\u5206\u6790\">\u9605\u8bfb\u66f4\u591a<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_crdt_document":"","footnotes":""},"categories":[6],"tags":[],"class_list":["post-1673","post","type-post","status-publish","format-standard","hentry","category-6"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>\u57fa\u56e0\u7ec4\u5355\u500d\u578b\u5206\u6790 - \u5c0f\u9a6c\u751f\u4fe1\u65e5\u8bb0<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/www.majunpeng.com\/index.php\/2025\/06\/13\/\u57fa\u56e0\u7ec4\u5355\u500d\u578b\u5206\u6790\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta 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